DNA adduct formation in mice following dermal application of smoke condensates from cigarettes that burn or heat tobacco

Journal Article · Wed Jan 01 00:00:00 EST 1992 · Environmental and Molecular Mutagenesis; (United States)

DOI:https://doi.org/10.1002/em.2850200410· OSTI ID:6387452

Lee, C K; Brown, B G; Reed, E A; Mosberg, A T; Doolittle, D J; Hayes, A W ^[1]; Hejtmancik, M ^[2]

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1. R.J. Reynolds Tobacco Co., Winston-Salem, NC (United States)
2. Battelle Columbus Lab., OH (United States)

A prototype cigarette that heats tobacco (test cigarette), developed by R.J. Reynolds Tobacco Company, has yielded consistently negative results in several in vivo and in vitro genetic toxicology tests. The objective of the present study was to evaluate the potential of cigarette smoke condensate (CSC) from the test cigarette to induce DNA adducts in mouse tissues and compare the results with those obtained with CSC from a reference tobacco-burning cigarette (1R4F). CD-1 mice were skin-painted with CSF from reference and test cigarettes three times a week for 4 weeks. The highest mass of CSC applied was 180 mg tar per week per animal for both reference and test cigarette. DNA adducts were analyzed in skin and lung tissues using the [sup 32]P-postlabeling method with the P[sub 1] nuclease modification. Distinct diagonal radioactive zones (DRZ) were observed in the DNA from both skin and lung tissues of animals dosed with reference CSC, whereas no corresponding DRZ were observed from the DNA of animals dosed with either test CSC or acetone (solvent control). The relative adduct labeling (RAL) values of skin and lung DNA from reference CSC-treated animals were significantly greater than those of the test CSC-treated animals. The RAL values of the test CSC-treated animals were no greater than those of solvent controls. The negative results in DNA adduct assays with test CSC are consistent with all previous results of in vivo and in vitro genetic toxicology testing on this cigarette and provide additional evidence that smoke condensate from the test cigarette is not genotoxic. 31 refs., 4 figs., 2 tabs.

• OSTI ID: 6387452
• Journal Information: Environmental and Molecular Mutagenesis; (United States), Vol. 20:4; ISSN 0893-6692
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
DNA ADDUCTS
SYNTHESIS
TOBACCO SMOKES
BIOLOGICAL EFFECTS
CARCINOGENS
CHRONIC EXPOSURE
LUNGS
MICE
MUTAGENESIS
MUTAGENS
MUTATIONS
RESPIRATORY TRACT CELLS
TOBACCO PRODUCTS
ADDUCTS
AEROSOLS
ANIMAL CELLS
ANIMALS
BODY
COLLOIDS
DISPERSIONS
MAMMALS
ORGANS
RESIDUES
RESPIRATORY SYSTEM
RODENTS
SMOKES
SOLS
VERTEBRATES
550400* - Genetics
560300 - Chemicals Metabolism & Toxicology