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Formation of cigarette smoke-induced DNA adducts in the rat lung and nasal mucosa

Technical Report ·
OSTI ID:6982888
; ;
The formation of DNA adducts in the nasal, lung, and liver tissues of rats exposed daily to fresh smoke from a University of Kentucky reference cigarette (2R1) for up to 40 weeks was examined. The amount of smoke total particulate matter (TPM) inhaled and the blood carboxyhemoglobin (COHb) values averaged 5-5.5 mg smoke TPM/day/rat and 5.5%, respectively. The pulmonary AHH activity measured at the termination of each experiment showed an average increase of about two- to threefold in smoke-exposed groups. These observations suggested that animals effectively inhaled both gaseous and particulate phase constituents of cigarette smoke. DNAs from nasal, lung, and liver tissue were extracted and analyzed by an improved {sup 32}P-postlabeling procedure. The data demonstrate the DNA-damaging potential of long term fresh cigarette smoke exposure and suggest the ability of the tissue to partially recover from such damage following cessation of the exposure.
Research Organization:
Baylor Coll. of Medicine, Houston, TX (USA). Dept. of Pharmacology
OSTI ID:
6982888
Report Number(s):
PB-90-140799/XAB
Country of Publication:
United States
Language:
English

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Related Subjects

550501 -- Metabolism-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADDUCTS
AEROSOLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BIOSYNTHESIS
BLOOD
BODY
BODY FLUIDS
CARBOXYHEMOGLOBIN
COLLOIDS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISPERSIONS
DNA ADDUCTS
DOSE-RESPONSE RELATIONSHIPS
GLANDS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIVER
LUNGS
MAMMALS
MATERIALS
NUCLEI
ODD-ODD NUCLEI
ORGANS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
RADIOISOTOPES
RATS
RESIDUES
RESPIRATION
RESPIRATORY SYSTEM
RODENTS
SMOKES
SOLS
SYNTHESIS
TOBACCO SMOKES
TOXICITY
TRACER TECHNIQUES
VERTEBRATES