Cigarette smoke-induced DNA adducts in the respiratory and nonrespiratory tissues of rats

Journal Article · Tue Jan 01 04:00:00 EST 1991 · Environmental and Molecular Mutagenesis; (United States)

DOI:https://doi.org/10.1002/em.2850170406· OSTI ID:5928387

Gairola, C G; Gupta, R C ^[1]

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1. Univ. of Kentucky, Lexington (United States)

Formation of DNA adducts is regarded as an essential initial step in the process of chemical carcinogenesis. To determine how chronic exposure to cigarette smoke affects the distribution of DNA adducts in selected respiratory and nonrespiratory tissues. The authors exposed male Sprague-Dawley rats daily to fresh mainstream smoke from the Univ. of Kentucky reference cigarettes (2R1) in a nose-only exposure system for 32 weeks. Blood carboxyhemoglobin, total particulate matter (TPM) intake, and pulmonary aryl hydrocarbon hydroxylase values indicated effective exposure of animals to cigarette smoke. DNA was extracted from three respiratory (larynx, trachea, and lung) and three nonrespiratory (liver, heart, and bladder) tissues and analyzed for DNA adducts by the {sup 32}P-postlabeling assay under conditions capable of detecting low levels of diverse aromatic/hydrophobic adducts. Data showed that the total DNA adducts in the lung, heart, and trachea, and larynx were increased by 10- to 20-fold in the smoke-exposed group. These data suggest selective formation of DNA adducts in the tissues.

• OSTI ID: 5928387
• Journal Information: Environmental and Molecular Mutagenesis; (United States), Journal Name: Environmental and Molecular Mutagenesis; (United States) Vol. 17:4; ISSN 0893-6692; ISSN EMMUE
• Country of Publication: United States
• Language: English

Related Subjects

550601 -- Medicine-- Unsealed Radionuclides in Diagnostics
560300* -- Chemicals Metabolism & Toxicology
62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADDUCTS
AEROSOLS
ANIMALS
AROMATICS
BIOLOGICAL EFFECTS
BLADDER
BODY
CARBOXYHEMOGLOBIN
CARCINOGENS
CARDIOVASCULAR SYSTEM
CHRONIC EXPOSURE
COLLOIDS
DIGESTIVE SYSTEM
DISPERSIONS
DISTRIBUTION
DNA ADDUCTS
ENZYMES
ESTERASES
GLANDS
HEART
HYDROLASES
LARYNX
LIVER
MAMMALS
NUCLEASES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANS
PHOSPHODIESTERASES
PROTEINS
RATS
RESIDUES
RESPIRATORY SYSTEM
RODENTS
SMOKES
SOLS
TISSUE DISTRIBUTION
TOBACCO SMOKES
TRACHEA
URINARY TRACT
VERTEBRATES