32P-postlabeling DNA adduct assay: cigarette smoke-induced dna adducts in the respiratory and nonrespiratory rat tissues. Book chapter
Technical Report
·
OSTI ID:5544968
An analysis of the tissue DNA adducts in rats by the sensitive (32)p-postlabeling assay showed one to eight detectable DNA adducts in lung, trachea, larynx, heart and bladder of the sham controls. Chronic exposure of animals to mainstream cigarette smoke showed a remarkable enhancement of most adducts in the lung and heart DNA. Since cigarette smoke contains several thousand chemicals and a few dozen of them are known or potential carcinogens, the difference between the DNA adducts of nasal and the other tissues may reflect the diversity of reactive constituents and their differential absorption in different tissues. In comparison to the lung DNA adducts, the adducts in nasal DNA were less hydrophobic. Identity of the predominant adducts was further investigated by comparison with several reference DNA adducts from 10 PAH and aromatic amines. Since some of these chemicals are present in cigarette smoke, the results suggest that these constituents of cigarette smoke may not be directly responsible for formation of DNA adducts in the lung and heart of the smoke-exposed animals.
- Research Organization:
- Kentucky Univ., Lexington, KY (USA). Graduate Center for Toxicology
- OSTI ID:
- 5544968
- Report Number(s):
- PB-91-162578/XAB
- Country of Publication:
- United States
- Language:
- English
Similar Records
Cigarette smoke-induced DNA adducts in the respiratory and nonrespiratory tissues of rats
Formation of cigarette smoke-induced DNA adducts in the rat lung and nasal mucosa
DNA adduct formation in mice following dermal application of smoke condensates from cigarettes that burn or heat tobacco
Journal Article
·
Mon Dec 31 23:00:00 EST 1990
· Environmental and Molecular Mutagenesis; (United States)
·
OSTI ID:5928387
Formation of cigarette smoke-induced DNA adducts in the rat lung and nasal mucosa
Technical Report
·
Sat Dec 31 23:00:00 EST 1988
·
OSTI ID:6982888
DNA adduct formation in mice following dermal application of smoke condensates from cigarettes that burn or heat tobacco
Journal Article
·
Tue Dec 31 23:00:00 EST 1991
· Environmental and Molecular Mutagenesis; (United States)
·
OSTI ID:6387452
Related Subjects
550501 -- Metabolism-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADDUCTS
AEROSOLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYHEMOGLOBIN
CARCINOGENS
COLLOIDS
COMBUSTION PRODUCTS
DAYS LIVING RADIOISOTOPES
DISPERSIONS
DISTRIBUTION
DNA ADDUCTS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
METABOLISM
MUTAGENS
NUCLEI
ODD-ODD NUCLEI
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
RADIOASSAY
RADIOISOTOPES
RATS
RESIDUES
RESPIRATORY SYSTEM
RODENTS
SMOKES
SOLS
TISSUE DISTRIBUTION
TOBACCO SMOKES
TRACER TECHNIQUES
VERTEBRATES
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADDUCTS
AEROSOLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYHEMOGLOBIN
CARCINOGENS
COLLOIDS
COMBUSTION PRODUCTS
DAYS LIVING RADIOISOTOPES
DISPERSIONS
DISTRIBUTION
DNA ADDUCTS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
METABOLISM
MUTAGENS
NUCLEI
ODD-ODD NUCLEI
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
RADIOASSAY
RADIOISOTOPES
RATS
RESIDUES
RESPIRATORY SYSTEM
RODENTS
SMOKES
SOLS
TISSUE DISTRIBUTION
TOBACCO SMOKES
TRACER TECHNIQUES
VERTEBRATES