Modification of the thermal response by D/sub 2/O: II. Thermotolerance and the specific inhibition of development

Journal Article · Wed Dec 01 04:00:00 EST 1982 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3575926· OSTI ID:6380255

Li, G C; Fisher, G A; Hahn, G M

In a companion paper it was shown that D/sub 2/O protects cells against the lethal effects of heat and that D/sub 2/O interfers with the induction of thermotolerance. In this paper we investigate the latter phenomenon with particular reference to an operational model of thermotolerance. Plateau-phase Chinese hamster ovary cells (HA-1) were treated at elevated temperatures permissive for the development of thermotolerance (40-42.5/sup 0/C) in media prepared with H/sub 2/O or D/sub 2/O. Cells were given a more lethal dose of heat (45/sup 0/C/45 min with surviving fraction approx. =10/sup -4/) to assess the degree of thermotolerance. Cells pretreated in H/sub 2/O media expressed a two to three log increase in survival, while thermotolerance was effectively suppressed in cells pretreated in D/sub 2/O. However, cells treated in D/sub 2/O media for 4 hr at 42/sup 0/C, then permitted a 37/sup 0/C incubation period in H/sub 2/O media, rapidly recovered their thermotolerance. Interpreting results within the framework of a three-component model of thermotolerance (trigger, development, decay), we suggest the following: (1) D/sub 2/O inhibits the development component of tolerance; (2) the effect of D/sub 2/O on the trigger component is such that the cells behave as if they were treated at a temperature lower by 2/sup 0/C in H/sub 2/O media; (3) the protection afforded by D/sub 2/O is mechanistically different from that of thermotolerance, at least in the sense that they augment each other.

• Research Organization: Stanford Univ. School of Medicine, CA
• OSTI ID: 6380255
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 92:3; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560122* -- Radiation Effects on Cells-- Internal Source-- (-1987)
560201 -- Thermal Effects-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY TEMPERATURE
CELL KILLING
CONNECTIVE TISSUE CELLS
DOSES
FIBROBLASTS
HAMSTERS
HEAVY WATER
HYDROGEN COMPOUNDS
HYPERTHERMIA
KINETICS
LETHAL DOSES
MAMMALS
OXYGEN COMPOUNDS
RADIATION EFFECTS
RODENTS
SOMATIC CELLS
SURVIVAL CURVES
TEMPERATURE EFFECTS
VERTEBRATES
WATER