Influence of hypoxia on the development of thermotolerance

Journal Article · Sat May 01 04:00:00 EDT 1982 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3575713· OSTI ID:5294500

Gerweck, L E; Bascomb, F

The effect of hypoxia on the lethal response of cells exposed to single or fractionated heat treatments was examined. Chinese hamster ovary cells were exposed to graded 43/sup 0/C heat treatments in medium equilibrated with air or nitrogen (25-40 ppm O/sub 2/) plus 5% CO/sub 2/ at pH 7.25-7.4. Hypoxia was induced approx. =0.5 hr prior to exposure of the cells to the single or initial heat treatment and was maintained until the second heat treatment was terminated. The response of cells to single heat treatments was not significantly different under oxic and hypoxic conditions, yielding D/sub 0/'s of 13.5 +/- 1.4 and 14.9 +/- 1.6 min, respectively. Following an initial heat treatment which reduced survival to 10%, cells became increasingly resistant, up to ninefold, to second heat treatments administered 0 to 12 hr later. Reduction in oxygen concentration during and between heat treatments affected neither the magnitude nor the kinetics of thermotolerance development.

• Research Organization: Harvard Medical School, Boston, MA
• OSTI ID: 5294500
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 90:2; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560121 -- Radiation Effects on Cells-- External Source-- (-1987)
560201* -- Thermal Effects-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANOXIA
BIOLOGICAL EFFECTS
BODY TEMPERATURE
CONNECTIVE TISSUE CELLS
DOSE-RESPONSE RELATIONSHIPS
ELEMENTS
FIBROBLASTS
HAMSTERS
HYPOTHERMIA
MAMMALS
NONMETALS
OXYGEN
RODENTS
SOMATIC CELLS
TEMPERATURE EFFECTS
VERTEBRATES