Influence of low pH on the development and decay of 42/sup 0/C thermotolerance in CHO cells

Journal Article · Mon Nov 01 04:00:00 EST 1982 · Int. J. Radiat. Oncol., Biol. Phys.; (United States)

DOI:https://doi.org/10.1016/0360-3016(82)90452-7· OSTI ID:5238375

Gerweck, L E; Richards, B; Michaels, H B

The development, magnitude and decay of thermotolerance in Chinese hamster ovary cells heated to 42/sup 0/C at pH 6.7 was examined. The cells were exposed to single or fractionated heat treatments with 0 to 168 hours elapsing between the treatments. Administration of a specified heat treatment in two fractions at low pH substantially reduced the lethal effect of heat. The rate of hyperthermic cell killing was reduced by a factor of approximately 2.5 in preheated cells compared to cells not receiving prior heat treatment. Tolerance to second heat treatments was apparent when 3 hours at 37/sup 0/C separated the treatments, and was near maximum when 6 hours separated the treatments. Beginning about 96 hours after the initial heat treatment, resistance to second heat treatments began to decline, and was not evident when 168 hours at 37/sup 0/C separated the treatments. In contrast to these results, a sparing effect as a result of dose fraction was not observed at pH 7.4. At pH 7.4, cells developed thermotolerance during the initial heat treatment and additional culturing at 37/sup 0/C was without additional effect. Nevertheless, the sensitivity of cells to hyperthermia was greater at pH 6.7 than at pH 7.4. This pH sensitizing effect was more pronounced in cells exposed to sinlge heat treatments than in cells exposed to fractionated treatments at 42/sup 0/C.

• Research Organization: Harvard Medical School, Boston, MA
• OSTI ID: 5238375
• Journal Information: Int. J. Radiat. Oncol., Biol. Phys.; (United States), Journal Name: Int. J. Radiat. Oncol., Biol. Phys.; (United States) Vol. 8:11; ISSN IOBPD
• Country of Publication: United States
• Language: English

Related Subjects

560201* -- Thermal Effects-- Cells-- (-1987)
560301 -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BODY
BODY TEMPERATURE
CELL KILLING
CHO CELLS
FEMALE GENITALS
GONADS
HAMSTERS
HYPERTHERMIA
MAMMALS
ORGANS
OVARIES
PH VALUE
RADIATION DOSE DISTRIBUTIONS
RODENTS
SENSITIVITY
TEMPORAL DOSE DISTRIBUTIONS
TOLERANCE
VERTEBRATES