Enhancement of mammalian cell sensitivity to hyperthermia by pH alteration

Journal Article · Wed Sep 01 04:00:00 EDT 1976 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3574347· OSTI ID:7145049

Gerweck, L; Rottinger, E

the effect of pH on the growth rate and reproductive capacity of Chinese hamster cells was examined at 37/sup 0/ and 42/sup 0/C. At 37/sup 0/C, the population doubling time did not vary between pH 7.4 and 7.0, but was prolonged at lower pH. Cell viability was not affected from pH 7.4 to 6.7. The effect of 42/sup 0/C on the viability of cells was strongly influenced by pH. Four hours of heat treatment resulted in a 10-fold decrease in survival at pH 7.0 and a 500-fold decrease in survival at pH 6.7, relative to pH 7.4. These results indicate that pH variations may play a role in determining sensitivity to hyperthermia. This pH effect may be significant under conditions which stimulate metabolic acidification.

• Research Organization: Massachusetts General Hospital, Boston
• OSTI ID: 7145049
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 67:3; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

550500* -- Metabolism
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BODY TEMPERATURE
CELL PROLIFERATION
HAMSTERS
HEAT TREATMENTS
HYPERTHERMIA
MAMMALS
PH VALUE
RODENTS
SURVIVAL TIME
VERTEBRATES