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Effect of hypoxia on recovery from damage induced by heat and radiation in plateau-phase CHO cells

Journal Article · · Radiat. Res.; (United States)
DOI:https://doi.org/10.2307/3576397· OSTI ID:5978775
;
The effect of hypoxia on the induction of and recovery from damage by radiation alone and in combination with heat has been investigated using plateau-phase Chinese hamster ovary (CHO) cells. Postirradiation hypoxia reduced the potentially lethal damage recovery (PLDR) in cells irradiated under an euoxic state and completely eliminated PLDR in cells irradiated under hypoxia. Cells which were maintained under hypoxia during both irradiation and a 4-hr recovery period and then incubated for a further period of 4 hr under euoxic conditions showed PLDR, suggesting that the inhibition of PLDR by hypoxia is reversible. Oligomycin completely eliminated PLDR when present during the postirradiation period. Pre- or postirradiation heat treatment at 42.5/sup 0/C for 30 min appreciably sensitized the cells to the induction of lethality. Thermal enhancement ratio (TER) was 1.7 for cells irradiated and heat treated under hypoxic conditions. The same heat treatment reduced the oxygen enhancement ratio (OER) associated with ..gamma.. radiation from 3.1 to 2.5. The kinetics of interaction between heat and radiation damage were studied by introducing a time gap of 4 hr between the treatments. Analysis of the results suggests that cells fail to recover from sublethal heat damage when held for 4 hr under hypoxic conditions. Cells held under hypoxic conditions partly recover from the radiation damage which subsequently interacts with sublethal heat damage, resulting in cell lethality.
Research Organization:
Medical Coll. of Wisconsin, Milwaukee
OSTI ID:
5978775
Journal Information:
Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 101:2; ISSN RAREA
Country of Publication:
United States
Language:
English

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Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
560201 -- Thermal Effects-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALI METAL ISOTOPES
ANIMAL CELLS
ANOXIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL RECOVERY
BODY TEMPERATURE
CELL CYCLE
CELL KILLING
CESIUM 137
CESIUM ISOTOPES
CHO CELLS
DATA
ELECTROMAGNETIC RADIATION
EXPERIMENTAL DATA
GAMMA RADIATION
HYPERTHERMIA
INFORMATION
IONIZING RADIATIONS
ISOTOPES
NUCLEI
NUMERICAL DATA
ODD-EVEN NUCLEI
RADIATIONS
RADIOINDUCTION
RADIOISOTOPES
RADIOSENSITIVITY EFFECTS
RECOVERY
YEARS LIVING RADIOISOTOPES