Modification of the heat response and thermotolerance by cycloheximide, hydroxyurea, and lucanthone in CHO cells

Journal Article · Fri Jan 01 04:00:00 EST 1982 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3575711· OSTI ID:5296622

Henle, K J ^[1]; Leeper, D B

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1. Univ. of Utah, Salt Lake City

Exposure of Chinese hamster ovary cells to cycloheximide for 2 hr immediately prior to 45/sup 0/C hyperthemia increased cell survival by a factor of 1.8. The increase in cell survival was independent of the heating time for heat treatments longer than 10 min at 45/sup 0/C and was similar with cycloheximide concentrations of 1 and 10 ..mu..g/ml. Thermotolerance was induced by an initial treatment of 10 min at 45/sup 0/C (conditioning), developed during a 7-hr incubation period at 37/sup 0/C, and was defined by the hyperthermia dose response with a second 45/sup 0/C heat treatment. When cycloheximide (1..mu..g/ml) was added to the medium after heat conditioning and removed prior to the second heat treatment, the degree of thermotolerance was 50% less than that in medium controls. A 3-hr exposure to 10 ..mu..g/ml cycloheximide at 37/sup 0/C by itself did not result in the progressive development of thermotolerance which occurs after a conditioning heat treatment. In contrast to the effects of cycloheximide, hydroxyurea (1 mM) and lucanthone (5 ..mu..g/ml) showed little effect on the heat sensitivity and the development of thermotolerance after heat conditioning. Although the results can be interpreted that the development of thermotolerance requires the synthesis of new proteins, but not that of DNA and RNA, alternate interpretations are possible based on known cycloheximide effects aside from its primary inhibition of protein synthesis.

• OSTI ID: 5296622
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 90:2; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMIDES
ANIMAL CELLS
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
BIOLOGICAL EFFECTS
BIOSYNTHESIS
BODY TEMPERATURE
CELL KILLING
CONNECTIVE TISSUE CELLS
CYCLOHEXIMIDE
DOSE-RESPONSE RELATIONSHIPS
DRUGS
FIBROBLASTS
FUNGICIDES
HAMSTERS
HYDROXY COMPOUNDS
HYDROXYUREA
HYPERTHERMIA
INHIBITION
MAMMALS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PESTICIDES
PROTEINS
RNA
RODENTS
SOMATIC CELLS
SYNTHESIS
TEMPERATURE EFFECTS
VERTEBRATES