Simultaneous determination of selenite and trimethylselenonium ions in urine by anion exchange chromatography and molecular neutron activation analysis
A method has been developed for the determination of trimethylselenonium (TMSe) ion and selenite (SeO/sub 3//sup 2 -/) ion in urine by anion exchange chromatography, selectively eluting TMSe and SeO/sub 3//sup 2 -/. Since the recoveries are quantitative, a method of additive spikes is employed to determine TMSe and SeO/sub 3//sup 2 -/ ion concentrations in urine specimens. The TMSe and SeO/sub 3//sup 2 -/ chromatographic elutions were collected in vials, irradiated with neutrons, and radioassayed for /sup 77m/Se activity. The limit of detection is 10 ng of Se as TMSe or SeO/sub 3//sup 2 -//ml of urine. Thirteen urine specimens from normal subjects were analyzed for TMSe, SeO/sub 3//sup 2 -/, and total selenium concentration.
- Research Organization:
- Veterans Administration Medical Center, Omaha, NE
- DOE Contract Number:
- FG02-84ER13231
- OSTI ID:
- 6337017
- Journal Information:
- Anal. Chem.; (United States), Journal Name: Anal. Chem.; (United States) Vol. 59:17; ISSN ANCHA
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ACTIVATION ANALYSIS
ANIONS
BARYON REACTIONS
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY FLUIDS
CHARGED PARTICLES
CHEMICAL ANALYSIS
CHROMATOGRAPHY
DATA
EXPERIMENTAL DATA
HADRON REACTIONS
INFORMATION
ION EXCHANGE CHROMATOGRAPHY
IONS
MATERIALS
NEUTRON REACTIONS
NUCLEAR REACTIONS
NUCLEON REACTIONS
NUMERICAL DATA
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
QUANTITATIVE CHEMICAL ANALYSIS
SELENITES
SELENIUM COMPOUNDS
SEPARATION PROCESSES
TRACE AMOUNTS
URINE
WASTES