Molecular neutron activation analysis of selenium metabolites in urine

Conference · Fri Jan 01 04:00:00 EST 1988 · Transactions of the American Nuclear Society; (USA)

OSTI ID:5546623

Blotcky, A J; Hansen, G T; Ebrahim, A; Rack, E P

Because of the biological importance of selenium in living biological systems, various analytical procedures have been developed for analysis of microquantities of elemental selenium, in urine, serum, and tissue. For urine selenium, these include atomic absorption spectrometry, solution absorption spectrometry, solution fluorescence spectrometry, volumetry, and neutron activation analysis. Of equal or greater importance is the determination of selenium metabolites present in urine for the purpose of describing the biological pathways for the metabolism of selenium in living organisms. While it is known from previous studies that trimethylselenonium ion (TMSe) is a major metabolite in urine, probably the result of reduction and methylation reaction, there are no definitive results in the literature indicating the nature or quantity of other selenium metabolic products in urine. Early techniques to measure TMSe levels in urine involved the use of the radiotracer {sup 75}Se. Because of the long biological half-life of selenium and issues of radiation exposure, its use in humans has been limited. In this paper, the authors report the experimental procedure for the determination of total selenoamino acid concentration in urine and present total selenium values, and, where applicable, TMSe, SeO{sup 2{minus}}{sub 3}, and total selenoamino acid concentrations in the urine of normal and diseased subjects.

• OSTI ID: 5546623
• Report Number(s): CONF-880601--
• Conference Information: Journal Name: Transactions of the American Nuclear Society; (USA) Journal Volume: 56
• Country of Publication: United States
• Language: English

Related Subjects

38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY
400700 -- Radiochemistry & Nuclear Chemistry
550500* -- Metabolism
59 BASIC BIOLOGICAL SCIENCES
ACTIVATION ANALYSIS
ANIMALS
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY FLUIDS
CHEMICAL ANALYSIS
ELEMENTS
MAMMALS
MAN
MATERIALS
METABOLISM
METABOLITES
PRIMATES
QUANTITY RATIO
SELENIUM
SEMIMETALS
URINE
VERTEBRATES
WASTES