Determination of selenium metabolites in biological fluids using instrumental and molecular neutron activation analysis

Journal Article · Thu Dec 15 04:00:00 EST 1988 · Anal. Chem.; (United States)

DOI:https://doi.org/10.1021/ac00175a019· OSTI ID:6191281

Blotcky, A J; Ebrahim, A; Rack, E P

Optimized procedures have been developed for the determination of total selenium, trimethylselenonium (TMSe) ion, and selenite (SeO/sub 3//sup 2/minus//) ion in urine and serum and for total selenoamino acids in urine by anion exchange chromatography and molecular neutron activation analysis. For urine samples containing greater than 40 ng of Se/mL, determination for TMSe and SeO/sub 3//sup 2/minus// ion can be performed. For urine samples containing nondetectable SeO/sub 3//sup 2/minus// ion whose concentration differential between the total selenium and TMSe is greater than or equal to 40 ng of Se/mL, total selenoamino acids can be determined by derivatization and anion exchange chromatography. For urine samples whose differential between total selenium and TMSe + SeO/sub 3//sup 2 -/ is greater than 80 ng of Se/mL, a precipitation/coprecipitation technique employing Ba(NO/sub 3/)/sub 2/ and (NH/sub 4/)/sub 2/SO/sub 4/ must be performed prior to the subsequent steps. The procedures were evaluated for over 100 specimens from normal and diseased subjects.

• Research Organization: Veterans Administration Medical Center, Omaha, NE (USA)
• DOE Contract Number: FG02-84ER13231
• OSTI ID: 6191281
• Journal Information: Anal. Chem.; (United States), Journal Name: Anal. Chem.; (United States) Vol. 60:24; ISSN ANCHA
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400101* -- Activation
Nuclear Reaction
Radiometric & Radiochemical Procedures
400105 -- Separation Procedures
550200 -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ACTIVATION ANALYSIS
ANIONS
BARYON REACTIONS
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BLOOD SERUM
BODY FLUIDS
CHARGED PARTICLES
CHEMICAL ANALYSIS
CHROMATOGRAPHY
DATA
ELEMENTS
EXPERIMENTAL DATA
HADRON REACTIONS
INFORMATION
ION EXCHANGE CHROMATOGRAPHY
IONS
MATERIALS
NEUTRON REACTIONS
NUCLEAR REACTIONS
NUCLEON REACTIONS
NUMERICAL DATA
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
QUANTITATIVE CHEMICAL ANALYSIS
SELENITES
SELENIUM
SELENIUM COMPOUNDS
SEMIMETALS
SEPARATION PROCESSES
URINE
WASTES