Immobilization of Aspergillus beta-glucosidase on Chitosan
Journal Article
·
· Appl. Environ. Microbiol.; (United States)
OSTI ID:6336357
..beta..-Glucosidase of Aspergillus phoenicis QM 329 was immobilized on chitosan, using the bifunctional agent glutaraldehyde. The most active preparation based on the amount of support contained a 1 : 2.5 enzyme-to-chitosan ratio (wt/wt). However, the specific activity of the bound enzyme decreased from 10 to 1% with increasing enzyme-to-chitosan ratio. Compared with free ..beta..-glucosidase, the immobilized enzyme exhibited: (1) a similar pH optimum but more activity at lower pH values; (2) improved thermal stability; (3) a similar response to inhibition by glucose; and (4) mass transfer limitations as reflected by higher apparent K/sub m/ and lower energy of activation.
- Research Organization:
- Army Natick Research and Development Command, Natick, MA
- OSTI ID:
- 6336357
- Journal Information:
- Appl. Environ. Microbiol.; (United States), Journal Name: Appl. Environ. Microbiol.; (United States) Vol. 35:4; ISSN AEMID
- Country of Publication:
- United States
- Language:
- English
Similar Records
Enzymatic hydrolysis of cellulose to glucose using immobilized /beta/-glucosidase
Immobilized and stabilized cellulase and. beta. -D-glucosidase
Utilization of immobilized B-glucosidase in the enzymatic hydrolysis of cellulose
Journal Article
·
Sat Feb 28 23:00:00 EST 1981
· Biotechnol. Bioeng.; (United States)
·
OSTI ID:5136238
Immobilized and stabilized cellulase and. beta. -D-glucosidase
Conference
·
Thu Dec 31 23:00:00 EST 1981
·
OSTI ID:5407149
Utilization of immobilized B-glucosidase in the enzymatic hydrolysis of cellulose
Thesis/Dissertation
·
Sat Dec 31 23:00:00 EST 1977
·
OSTI ID:5974651