Immobilized and stabilized cellulase and. beta. -D-glucosidase
The major types of components of cellulase from Trichoderma reesei were adsorbed onto Concanavalin A immobilized on Sepharose 4B suggesting that they are glycoproteins. These components were covalently coupled to cyanogen bromide-activated Sepharose after aminoalkylation of their periodate-oxidized carbohydrate side chains to provide additional points of attachment of the enzyme to the support. Although there was only a 9% recovery of starting avicelase activity, the immobilzed enzyme catalyzed the hydrolysis of insoluble cellulose to glucose with greater efficiency than did free cellulase. The enzyme ..beta..-D-glucosidase from Aspergillus niger was also immobilized through its carbohydrate moiety by these methods. An increase in the thermal stability of the immobilized ..beta..-D-glucosidase preparations over the soluble enzyme was achieved if the enzyme was treated with glutaraldehyde prior to its adsorption on concanavalin A-Sepharose or if the enzyme immobilized on cyanogen bromide-activated Sepharose was subsequently treated with glutaraldehyde.
- Research Organization:
- Oak Ridge National Lab., TN (USA)
- DOE Contract Number:
- W-7405-ENG-26
- OSTI ID:
- 5407149
- Report Number(s):
- CONF-820202-8; ON: DE82011440
- Resource Relation:
- Conference: AICHE 1982 national winter meeting, Orlando, FL, USA, 28 Feb 1982
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
CELLULASE
IMMOBILIZED ENZYMES
GLUCOSIDASE
CELLULOLYTIC ACTIVITY
ASPERGILLUS
CELLULOSE
CONCANAVALIN
ENZYMATIC HYDROLYSIS
ENZYME ACTIVITY
EXPERIMENTAL DATA
GLUCOPROTEINS
STABILITY
TEMPERATURE DEPENDENCE
TRICHODERMA
AGGLUTININS
ANTIBODIES
CARBOHYDRATES
CHEMICAL REACTIONS
DATA
DECOMPOSITION
ENZYMES
FUNGI
GLYCOSYL HYDROLASES
HEMAGGLUTININS
HYDROLASES
HYDROLYSIS
INFORMATION
LYSIS
NUMERICAL DATA
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
PLANTS
POLYSACCHARIDES
PROTEINS
SACCHARIDES
SOLVOLYSIS
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