Immobilized and stabilized cellulase and. beta. -D-glucosidase
The major types of components of cellulase from Trichoderma reesei were adsorbed onto Concanavalin A immobilized on Sepharose 4B suggesting that they are glycoproteins. These components were covalently coupled to cyanogen bromide-activated Sepharose after aminoalkylation of their periodate-oxidized carbohydrate side chains to provide additional points of attachment of the enzyme to the support. Although there was only a 9% recovery of starting avicelase activity, the immobilzed enzyme catalyzed the hydrolysis of insoluble cellulose to glucose with greater efficiency than did free cellulase. The enzyme ..beta..-D-glucosidase from Aspergillus niger was also immobilized through its carbohydrate moiety by these methods. An increase in the thermal stability of the immobilized ..beta..-D-glucosidase preparations over the soluble enzyme was achieved if the enzyme was treated with glutaraldehyde prior to its adsorption on concanavalin A-Sepharose or if the enzyme immobilized on cyanogen bromide-activated Sepharose was subsequently treated with glutaraldehyde.
- Research Organization:
- Oak Ridge National Lab., TN (USA)
- DOE Contract Number:
- W-7405-ENG-26
- OSTI ID:
- 5407149
- Report Number(s):
- CONF-820202-8; ON: DE82011440
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
AGGLUTININS
ANTIBODIES
ASPERGILLUS
CARBOHYDRATES
CELLULASE
CELLULOLYTIC ACTIVITY
CELLULOSE
CHEMICAL REACTIONS
CONCANAVALIN
DATA
DECOMPOSITION
ENZYMATIC HYDROLYSIS
ENZYME ACTIVITY
ENZYMES
EXPERIMENTAL DATA
FUNGI
GLUCOPROTEINS
GLUCOSIDASE
GLYCOSYL HYDROLASES
HEMAGGLUTININS
HYDROLASES
HYDROLYSIS
IMMOBILIZED ENZYMES
INFORMATION
LYSIS
NUMERICAL DATA
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
PLANTS
POLYSACCHARIDES
PROTEINS
SACCHARIDES
SOLVOLYSIS
STABILITY
TEMPERATURE DEPENDENCE
TRICHODERMA