Utilization of immobilized B-glucosidase in the enzymatic hydrolysis of cellulose
- Univ. of California, Berkeley, CA (United States)
β-glucosidase obtained from Aspergillus phoenicis was immobilized onto phenol formaldehyde resin using glutaraldehyde as a fixing agent, and kinetic characteristics such as pH optimum, temperature stability and Michaelis-Menton constants were determined. Three experiments were performed where a batch hydrolysis of a cellulosic source was carried out with a recycle stream through an immobilized β-glucosidase column in order to continuously remove cellobiose. The first two experiments using pretreated corn stover as the substrate showed no increase in hydrolysis over that of a control system, presumably because the cellobiose production was too low for cellobiose inhibition to occur. The third experiment, using Solka Floc as the substrate, which produced as high as 8.8 grams per liter of cellobiose, showed only a slight increase in soluble sugar production over that of the control system. Since the current process indicates the use of corn stover or a similar substrate, it does not appear useful to include an immobilized enzyme reactor in this manner. Since the fermentation part of the process cannot use cellobiose to produce ethanol, the use of the immobilized β-glucosidase reactor to convert the cellobiose to glucose may have economic significance by increasing the ethanol yield in this fashion. A computer program was produced in order to simulate a fixed-bed reactor with diffusion limitations and to determine the cost per pound of glucose for a given reactor design. Use of the immobilized enzyme system results in a savings of 0.53 cents per pound of glucose, which results in a corresponding savings of 7.2 cents per gallon of ethanol upon subsequent fermentation of the hydrolyzate.
- Research Organization:
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- W-7405-ENG-48
- OSTI ID:
- 5974651
- Report Number(s):
- LBL--7857
- Country of Publication:
- United States
- Language:
- English
Similar Records
Enzymatic hydrolysis of cellulose to glucose using immobilized /beta/-glucosidase
Improvement of yields and rates during enzymatic hydrolysis of cellulose to glucose. Final report, December 1, 1978-January 31, 1980
Related Subjects
090222* -- Alcohol Fuels-- Preparation from Wastes or Biomass-- (1976-1989)
140504 -- Solar Energy Conversion-- Biomass Production & Conversion-- (-1989)
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
ALCOHOLS
ALDEHYDES
ASPERGILLUS
BIOSYNTHESIS
CARBOHYDRATES
CELLOBIOSE
CELLULOLYTIC ACTIVITY
CHEMICAL REACTIONS
DECOMPOSITION
DISACCHARIDES
ECONOMICS
ENZYMATIC HYDROLYSIS
ENZYME ACTIVITY
ENZYMES
ETHANOL
FUNGI
GLUCOSE
GLUCOSIDASE
GLYCOSYL HYDROLASES
HEXOSES
HYDROLASES
HYDROLYSIS
HYDROXY COMPOUNDS
IMMOBILIZED ENZYMES
LYSIS
MONOSACCHARIDES
O-GLYCOSYL HYDROLASES
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
PLANTS
SACCHARIDES
SOLVOLYSIS
SYNTHESIS