Enzymatic hydrolysis of cellulose to glucose using immobilized /beta/-glucosidase

Journal Article · Sun Mar 01 00:00:00 EST 1981 · Biotechnol. Bioeng.; (United States)

DOI:https://doi.org/10.1002/bit.260230302· OSTI ID:5136238

Sundstrom, D W; Klei, H E; Coughlin, R W; Biederman, G J; Brouwer, C A

This research investigated the use of supplemental immobilized /beta/-glucosidase to increase yields of glucose. Immobilized /beta/-glucosidase from Aspergillus phoenicis was produced by sorption on controlled-pore alumina with about 90% activity retention. The product lost only about 10% of the original activity during an on-stream reaction period of 500 hr with cellobiose as substrate; maximum activity occurred near pH 3.5 and the apparent activation energy was about 11 kcal/mol. The immobilized /beta/-glucosidase was used together with Trichoderma reesei cellulase to hydrolyze cellulosic materials, such as Solka Floc, corn stover, and exploded wood. Increased yields of glucose and greater conversions of cellobiose to glucose were observed when the reaction systems contained supplemental immobilized /beta/-glucosidase.

• Research Organization: Univ. of Connecticut, Storrs
• OSTI ID: 5136238
• Journal Information: Biotechnol. Bioeng.; (United States), Vol. 23:3
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CELLULOSE
ENZYMATIC HYDROLYSIS
GLUCOSE
PRODUCTION
BIOMASS
CELLOBIOSE
CELLULASE
GLUCOSIDASE
IMMOBILIZED ENZYMES
TRICHODERMA
ALDEHYDES
CARBOHYDRATES
CHEMICAL REACTIONS
DECOMPOSITION
DISACCHARIDES
ENERGY SOURCES
ENZYMES
FUNGI
GLYCOSYL HYDROLASES
HEXOSES
HYDROLASES
HYDROLYSIS
LYSIS
MONOSACCHARIDES
O-GLYCOSYL HYDROLASES
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
PLANTS
POLYSACCHARIDES
RENEWABLE ENERGY SOURCES
SACCHARIDES
SOLVOLYSIS
550200* - Biochemistry