Enzymatic hydrolysis of cellulose to glucose using immobilized /beta/-glucosidase

Journal Article · Sun Mar 01 04:00:00 EST 1981 · Biotechnol. Bioeng.; (United States)

DOI:https://doi.org/10.1002/bit.260230302· OSTI ID:5136238

Sundstrom, D W; Klei, H E; Coughlin, R W; Biederman, G J; Brouwer, C A

This research investigated the use of supplemental immobilized /beta/-glucosidase to increase yields of glucose. Immobilized /beta/-glucosidase from Aspergillus phoenicis was produced by sorption on controlled-pore alumina with about 90% activity retention. The product lost only about 10% of the original activity during an on-stream reaction period of 500 hr with cellobiose as substrate; maximum activity occurred near pH 3.5 and the apparent activation energy was about 11 kcal/mol. The immobilized /beta/-glucosidase was used together with Trichoderma reesei cellulase to hydrolyze cellulosic materials, such as Solka Floc, corn stover, and exploded wood. Increased yields of glucose and greater conversions of cellobiose to glucose were observed when the reaction systems contained supplemental immobilized /beta/-glucosidase.

• Research Organization: Univ. of Connecticut, Storrs
• OSTI ID: 5136238
• Journal Information: Biotechnol. Bioeng.; (United States), Journal Name: Biotechnol. Bioeng.; (United States) Vol. 23:3; ISSN BIBIA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
BIOMASS
CARBOHYDRATES
CELLOBIOSE
CELLULASE
CELLULOSE
CHEMICAL REACTIONS
DECOMPOSITION
DISACCHARIDES
ENERGY SOURCES
ENZYMATIC HYDROLYSIS
ENZYMES
FUNGI
GLUCOSE
GLUCOSIDASE
GLYCOSYL HYDROLASES
HEXOSES
HYDROLASES
HYDROLYSIS
IMMOBILIZED ENZYMES
LYSIS
MONOSACCHARIDES
O-GLYCOSYL HYDROLASES
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
PLANTS
POLYSACCHARIDES
PRODUCTION
RENEWABLE ENERGY SOURCES
SACCHARIDES
SOLVOLYSIS
TRICHODERMA