Improvement of yields and rates during enzymatic hydrolysis of cellulose to glucose. Progress report, June 1-August 31, 1979

Technical Report · Mon Oct 01 04:00:00 EDT 1979

DOI:https://doi.org/10.2172/5649553· OSTI ID:5649553

Sundstrom, D W; Klei, H E; Coughlin, R W

During this quarter experiments were conducted on enzymatic hydrolysis of cellulose using T. reesei cellulase together with the immobilized ..beta..-glucosidase (..beta..-G) described in earlier reports. In batch, slurry, and fluidized bed reactors, the presence of the immobilized ..beta..-G reduced cellobiose concentrations to low levels, such that glucose yields were increased by over 100% with Solka floc and corn stover as substrates. Acid pretreated corn stover from the Dartmouth group was readily hydrolyzed by cellulase when combined with our immobilized ..beta..-G, giving glucose yields comparable to that from Solka flox, based on cellulose content. These studies complete the major tasks outlined in our original proposal. During the past twelve months: microbial ..beta..-glucosidase (..beta..-G) from A. phoenicis has been produced and purified; the (..beta..-G) has been immobilized on an alumina support yielding long half lives and having immobilization activities above 80%; The immobilized ..beta..-G in several enzymatic reaction systems has been tested using a practical cellulose source (corn stover); and glucose yield has been increased by over 100% by employing the immobilized ..beta..-G. This promising enzyme reaction system will be developed further under funding based on a renewal application already submitted to SERI.

• Research Organization: Connecticut Univ., Storrs (USA)
• Sponsoring Organization: USDOE
• OSTI ID: 5649553
• Report Number(s): COO-5141-3
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ACID HYDROLYSIS
AGRICULTURAL WASTES
ALDEHYDES
BATCH CULTURE
BIOSYNTHESIS
CARBOHYDRATES
CELLULASES
CELLULOSE
CHEMICAL REACTION YIELD
CHEMICAL REACTIONS
DECOMPOSITION
DISPERSIONS
ENZYMATIC HYDROLYSIS
ENZYMES
FLUIDIZED BED
FUNGI
GLUCOSE
HEXOSES
HYDROLASES
HYDROLYSIS
IMMOBILIZED ENZYMES
LYSIS
MIXTURES
MONOSACCHARIDES
ORGANIC COMPOUNDS
PLANTS
POLYSACCHARIDES
SACCHARIDES
SLURRIES
SOLID WASTES
SOLVOLYSIS
SUSPENSIONS
SYNTHESIS
TRICHODERMA
WASTES
YIELDS