Enzymic hydrolysis of cellulose to glucose using immobilized b-glucosidase
The production of sugars by enzymic hydrolysis of cellulose is a multistep process which includes conversion of the intermediate cellobiose to glucose by B-glucosidase. Aside from its role as an intermediate cellobiose inhibits the endolglucanase components of typical cellulase enzyme systems. Because these enzyme systems often contain insufficient concentrations of B-glucosidase to prevent accumulation of inhibitory cellobiose, the use of supplemental immobilized B-glucosidase to increase yields of glucose was investigated. Immobolized B-glucosidase from Aspergillus phoenicis was produced by sorption on controlled-pore alumina with approximately 90% activity retention. The product lost only approximately 10% of the original activity during an on-stream reaction period of 500 h with cellobiose as substrate. Maximum activity occurred near pH 3.5, and the apparent activation energy was approx. 11 kcal/mol. The immobilized B-glucosidase was used together with Trichoderma reesei cellulase to hydrolyze cellulosic materials, such as Solka Floc, corn stover, and exploded wood. Increased yields of glucose and greater conversion of cellobiose to glucose were observed when the reaction systems contained supplemental immobilized B-glucosidase.
- Research Organization:
- Dept. Chem. Eng., Univ. Connecticut, Storrs, CT 06268, USA
- OSTI ID:
- 5462001
- Journal Information:
- Biotechnol. Bioeng.; (United States), Journal Name: Biotechnol. Bioeng.; (United States) Vol. 23:3; ISSN BIBIA
- Country of Publication:
- United States
- Language:
- English
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Improvement of yields and rates during enzymatic hydrolysis of cellulose to glucose. Final report, December 1, 1978-January 31, 1980
Utilization of immobilized B-glucosidase in the enzymatic hydrolysis of cellulose
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59 BASIC BIOLOGICAL SCIENCES
AGRICULTURAL WASTES
ALDEHYDES
ASPERGILLUS
CARBOHYDRATES
CELLOBIOSE
CELLULASE
CELLULOSE
CEREALS
CHEMICAL REACTIONS
DECOMPOSITION
DISACCHARIDES
ENZYMATIC HYDROLYSIS
ENZYMES
FUNGI
GLUCOSE
GLUCOSIDASE
GLYCOSYL HYDROLASES
GRAMINEAE
GRASS
HEXOSES
HYDROLASES
HYDROLYSIS
IMMOBILIZED ENZYMES
INHIBITION
LYSIS
MAIZE
MONOSACCHARIDES
O-GLYCOSYL HYDROLASES
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
ORGANIC WASTES
PLANTS
POLYSACCHARIDES
SACCHARIDES
SOLVOLYSIS
TRICHODERMA
TRICHODERMA VIRIDE
WASTES
WOOD