Enzymic hydrolysis of cellulose to glucose using immobilized b-glucosidase
The production of sugars by enzymic hydrolysis of cellulose is a multistep process which includes conversion of the intermediate cellobiose to glucose by B-glucosidase. Aside from its role as an intermediate cellobiose inhibits the endolglucanase components of typical cellulase enzyme systems. Because these enzyme systems often contain insufficient concentrations of B-glucosidase to prevent accumulation of inhibitory cellobiose, the use of supplemental immobilized B-glucosidase to increase yields of glucose was investigated. Immobolized B-glucosidase from Aspergillus phoenicis was produced by sorption on controlled-pore alumina with approximately 90% activity retention. The product lost only approximately 10% of the original activity during an on-stream reaction period of 500 h with cellobiose as substrate. Maximum activity occurred near pH 3.5, and the apparent activation energy was approx. 11 kcal/mol. The immobilized B-glucosidase was used together with Trichoderma reesei cellulase to hydrolyze cellulosic materials, such as Solka Floc, corn stover, and exploded wood. Increased yields of glucose and greater conversion of cellobiose to glucose were observed when the reaction systems contained supplemental immobilized B-glucosidase.
- Research Organization:
- Dept. Chem. Eng., Univ. Connecticut, Storrs, CT 06268, USA
- OSTI ID:
- 5462001
- Journal Information:
- Biotechnol. Bioeng.; (United States), Vol. 23:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
CELLULOSE
ENZYMATIC HYDROLYSIS
GLUCOSIDASE
IMMOBILIZED ENZYMES
AGRICULTURAL WASTES
ASPERGILLUS
CELLOBIOSE
CELLULASE
GLUCOSE
INHIBITION
MAIZE
SACCHARIDES
TRICHODERMA VIRIDE
WOOD
ALDEHYDES
CARBOHYDRATES
CEREALS
CHEMICAL REACTIONS
DECOMPOSITION
DISACCHARIDES
ENZYMES
FUNGI
GLYCOSYL HYDROLASES
GRAMINEAE
GRASS
HEXOSES
HYDROLASES
HYDROLYSIS
LYSIS
MONOSACCHARIDES
O-GLYCOSYL HYDROLASES
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
ORGANIC WASTES
PLANTS
POLYSACCHARIDES
SOLVOLYSIS
TRICHODERMA
WASTES
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