Concentration-dependent inhibition of development of GGT positive foci in rat liver by the environmental contaminant di(2-ethylhexyl) phthalate
The ability of di(2-ethylhexyl) phthalate (DEHP), a widely used plasticizer and environmental contaminant, to suppress development of putative preneoplastic lesions in rat liver was evaluated. el-Glutamyl transpeptidase-positive (GGT+) foci were initiated in the livers of Sprague-Dawley male rats with a single dose of diethylnitrosamine (DEN) following partial hepatectomy. Promotion of foci was commenced by feeding a choline-deficient diet (CD). A group of control rats was fed a choline-supplemented diet (CS). The ability of DEHP to suppress the emergence of GGT+ foci was evaluated by feeding additional groups of rats the CD diet containing either 0.1%, 0.5%, 1.0% or 2.0% DEHP. The CD diet promoted the number of GGT+ foci above levels in control livers. Inclusion of the plasticizer to the levels of 0.5%, 1.0% and 2.0% in the CD diet effectively inhibited the appearance of the foci. However, DEHP was unable to inhibit the promoting effect of the CD diet at a concentration of 0.1%. DEHP's ability to block development of GGT+ foci correlated with its ability to increase liver weight and to induce carnitine acetyltransferase (EC 2.3.1.7), a marker of peroxisome proliferation.
- Research Organization:
- George Washington Univ., Washington, DC
- OSTI ID:
- 6330943
- Journal Information:
- Environ. Health Perspect.; (United States), Vol. 60
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ENZYME ACTIVITY
INHIBITION
PHTHALIC ACID ESTERS
TOXICITY
TRANSFERASES
CHOLINE
DOSE-RESPONSE RELATIONSHIPS
EXPERIMENTAL DATA
LIVER
NITROSAMINES
RATS
RESPONSE MODIFYING FACTORS
ALCOHOLS
AMINES
AMMONIUM COMPOUNDS
ANIMALS
BODY
DATA
DIGESTIVE SYSTEM
DRUGS
ENZYMES
ESTERS
GLANDS
HYDROXY COMPOUNDS
INFORMATION
LIPOTROPIC FACTORS
MAMMALS
NITROSO COMPOUNDS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
QUATERNARY COMPOUNDS
RODENTS
VERTEBRATES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)