Effects of high dietary sucrose on the development of enzyme-altered foci during chemical hepatocarcinogenesis
Dietary factors may influence initiation, promotion or progression of tumors. To examine whether a high sucrose diet has any effect on the development of enzyme-altered foci (EAF) during promotion of hepatocarcinogenesis, one day old Sprague-Dawley rats were given a single i.p. dose of diethylnitrosamine (DEN). Controls received an equivalent i.p. volume of saline. After weaning at 21 days, rats were fed modified AIN-76 diets containing either 65% glucose (HGD) or sucrose (HSD), with or without 0.05% phenobarbital (PB). Four weeks later females on HSD, but not males, had significantly heavier livers than those of HGD. Female rats fed HSD developed twice as many ..gamma..-glutamyl transpeptidase positive foci/cm/sup 2/ of liver sections than animals on HGD. Addition of PB increased the number of foci in the HGD group five-fold and, in the HSD group, three-fold. No significant difference was found between the number of foci in animals on either of the two PB-supplemented diets. Results in males were similar, although differences between treatments were smaller. By autoradiography it was shown that hepatocytes within EAF, both in females and in males, had significantly higher DNA synthesizing activity than surrounding normal hepatocytes. These results suggest that HSD has a weak promoting effect during DEN-hepatocarcinogenesis in rats.
- OSTI ID:
- 5998884
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
LIVER
NEOPLASMS
NITROSAMINES
TOXICITY
SACCHAROSE
CARCINOGENESIS
DIET
ENZYME ACTIVITY
ETIOLOGY
GLUCOSE
NEONATES
PEPTIDE HYDROLASES
RATS
SEX DEPENDENCE
TUMOR PROMOTERS
ALDEHYDES
AMINES
ANIMALS
BODY
CARBOHYDRATES
DIGESTIVE SYSTEM
DISACCHARIDES
DISEASES
ENZYMES
GLANDS
HEXOSES
HYDROLASES
MAMMALS
MONOSACCHARIDES
NITROSO COMPOUNDS
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PATHOGENESIS
PROMOTERS
RODENTS
SACCHARIDES
VERTEBRATES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)