Dietary effects on initiation and promotion during hepatocarcinogenesis in the rat
Female F344/N rats were initiated with diethylnitrosamine (10 mg/kg) 24 hours following a 70% partial hepatectomy. They were fed for 1 week on diets without added promoting agents and then placed on a cereal-based NIH-07 diet, an AIN semipurified diet, or a semipurified diet described by Pariza (PD) for varying periods of time in the presence and absence of the promoting agent, phenobarbital (PB). After 6 months on the diets animals were sacrificed and serial frozen sections of the liver stained for gamma-glutamyltranspeptidase (GGT), canalicular ATPase, and glucose-6-phosphatase. The number and phenotype of altered foci (AF) were determined by quantitative stereology. Livers from rats fed the AIN or PD diets + PB after the first week post-initiation had fewer AF than groups fed the NIH-07 + PB. Livers of rats fed semipurified diets exhibited little periportal GGT staining, while such was extensive in livers of animals fed the NIH-07 diet. Livers of animals receiving the NIH-07 diet from the time of initiation exhibited significantly more AF than those fed PB with semipurified diets. Rats initiated on the semipurified diets and transferred to the NIH-07 diet + PB exhibited the highest number of AF. These studies indicate that cereal-based diets contain factors which inhibit initiation but act to enhance promotion during hepatocarcinogenesis.
- Research Organization:
- Univ. of Wisconsin, Madison
- OSTI ID:
- 6962318
- Report Number(s):
- CONF-8604222-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:4; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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NITROSAMINES
CARCINOGENESIS
PHENOBARBITAL
ATP-ASE
DIET
HEPATECTOMY
LIVER
PEPTIDE HYDROLASES
PHOSPHATES
RATS
ACID ANHYDRASES
AMINES
ANESTHETICS
ANIMALS
ANTICONVULSANTS
AZINES
BARBITURATES
BODY
CENTRAL NERVOUS SYSTEM DEPRESSANTS
DIGESTIVE SYSTEM
DRUGS
ENZYMES
GLANDS
HETEROCYCLIC COMPOUNDS
HYDROLASES
HYPNOTICS AND SEDATIVES
MAMMALS
MEDICINE
NITROSO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PATHOGENESIS
PHOSPHOHYDROLASES
PHOSPHORUS COMPOUNDS
PYRIMIDINES
RODENTS
SURGERY
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology