Dietary effects on initiation and promotion during hepatocarcinogenesis in the rat

Conference · Wed Mar 05 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6962318

Hendrich, S; Pitot, H C

Female F344/N rats were initiated with diethylnitrosamine (10 mg/kg) 24 hours following a 70% partial hepatectomy. They were fed for 1 week on diets without added promoting agents and then placed on a cereal-based NIH-07 diet, an AIN semipurified diet, or a semipurified diet described by Pariza (PD) for varying periods of time in the presence and absence of the promoting agent, phenobarbital (PB). After 6 months on the diets animals were sacrificed and serial frozen sections of the liver stained for gamma-glutamyltranspeptidase (GGT), canalicular ATPase, and glucose-6-phosphatase. The number and phenotype of altered foci (AF) were determined by quantitative stereology. Livers from rats fed the AIN or PD diets + PB after the first week post-initiation had fewer AF than groups fed the NIH-07 + PB. Livers of rats fed semipurified diets exhibited little periportal GGT staining, while such was extensive in livers of animals fed the NIH-07 diet. Livers of animals receiving the NIH-07 diet from the time of initiation exhibited significantly more AF than those fed PB with semipurified diets. Rats initiated on the semipurified diets and transferred to the NIH-07 diet + PB exhibited the highest number of AF. These studies indicate that cereal-based diets contain factors which inhibit initiation but act to enhance promotion during hepatocarcinogenesis.

• Research Organization: Univ. of Wisconsin, Madison
• OSTI ID: 6962318
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:4
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACID ANHYDRASES
AMINES
ANESTHETICS
ANIMALS
ANTICONVULSANTS
ATP-ASE
AZINES
BARBITURATES
BODY
CARCINOGENESIS
CENTRAL NERVOUS SYSTEM DEPRESSANTS
DIET
DIGESTIVE SYSTEM
DRUGS
ENZYMES
GLANDS
HEPATECTOMY
HETEROCYCLIC COMPOUNDS
HYDROLASES
HYPNOTICS AND SEDATIVES
LIVER
MAMMALS
MEDICINE
NITROSAMINES
NITROSO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PATHOGENESIS
PEPTIDE HYDROLASES
PHENOBARBITAL
PHOSPHATES
PHOSPHOHYDROLASES
PHOSPHORUS COMPOUNDS
PYRIMIDINES
RATS
RODENTS
SURGERY
VERTEBRATES