Trichloroacetic acid effects on rat liver peroxisomes and enzyme-altered foci

Journal Article · Sat Nov 01 04:00:00 EST 1986 · Environ. Health Perspect.; (United States)

DOI:https://doi.org/10.1289/ehp.866973· OSTI ID:5554963

Parnell, M J; Koller, L D; Exon, J H; Arnzen, J M

The initiating and promoting effects of trichloroacetic acid (TCA) were investigated using a rat hepatic enzyme-altered foci bioassay. The experimental protocol used has been shown to induce ..gamma..-glutamyltranspeptidase (GGT)-positive foci in hepatic tissue following an initiating dose with a genotoxic carcinogen. Twenty-four hours following 2/3 partial hepatectomy, rats received either a single oral dose (1500 mg/kg) or 5000 ppm TCA in drinking water for 10, 20, or 30 days. Two weeks after the end of TCA exposure, the rats were promoted for 3 or 6 months with 500 ppm phenobarbital in drinking water. TCA failed to induce GGT-positive foci using this initiation protocol. In addition, groups of 2/3 partially hepatectomized rats were initiated with a single oral dose of diethylnitrosamine (10 mg/kg) and then administered 50, 500, or 5000 ppm TCA drinking water. In this promotion protocol, TCA exposure resulted in a significant increase in the number of GGT-positive foci. The ability of TCA to stimulate peroxisomal-dependent palmitoyl-coenzyme A oxidation was also investigated. The findings support the hypothesis that TCA may possess weak promoting activity in the rat liver.

• Research Organization: Univ. of Idaho, Moscow
• OSTI ID: 5554963
• Journal Information: Environ. Health Perspect.; (United States), Journal Name: Environ. Health Perspect.; (United States) Vol. 69; ISSN EVHPA
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACETIC ACID
AMINES
ANIMALS
BIOLOGICAL EFFECTS
BODY
CARBOXYLIC ACIDS
CARCINOGENESIS
CHEMICAL REACTIONS
CHLORINATED ALIPHATIC HYDROCARBONS
DIGESTIVE SYSTEM
DRINKING WATER
ENZYME ACTIVITY
ENZYMES
GLANDS
HALOGENATED ALIPHATIC HYDROCARBONS
HYDROGEN COMPOUNDS
HYDROLASES
LIVER
MAMMALS
MONOCARBOXYLIC ACIDS
NITROSAMINES
NITROSO COMPOUNDS
ORAL ADMINISTRATION
ORGANIC ACIDS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
OXIDATION
OXYGEN COMPOUNDS
PATHOGENESIS
PEPTIDE HYDROLASES
PROMOTERS
RATS
RODENTS
TUMOR PROMOTERS
VERTEBRATES
WATER