Lack of significant promoting activity by benzene in the rat liver model of carcinogenesis

Journal Article · Tue Aug 01 04:00:00 EDT 1995 · Journal of Toxicology and Environmental Health

DOI:https://doi.org/10.1080/15287399509532010· OSTI ID:183517

Taningher, M; Perrotta, A; Malacarne, D ^[1]

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1. Interuniversitario per la Ricerca sul Cancro, Genoa (Italy); and others

The promoting activity of benzene on rat liver carcinogenesis was investigated. The chemical was tested for its ability to enhance the growth of preneoplastic foci, as detected by gamma-glutamyl transpeptidase (GGT) staining in diethylnitrosamine (DENA) initiated hepatocytes. Two weeks after receiving a single ip dose of 200 mg/kg DENA, F344 rats were given daily oral doses of 400 mg/kg benzene (5 d/wk) for 6 wk. At wk 3 after the experiment began, all animals underwent partial hepatectomy, and at wk 8 were sacrificed. Following benzene treatment, no variation in the liver/body weight ratio was observed. After scoring of foci in liver slides, no significant difference in foci number and area could be observed between rats treated with DENA plus benzene and rats treated with DENA alone. Practically no foci were observed in the liver of rats treated only with benzene. The lack of benzene promoting activity in the liver model is discussed. 28 refs., 1 fig., 1 tab.

• Sponsoring Organization: USDOE
• OSTI ID: 183517
• Journal Information: Journal of Toxicology and Environmental Health, Journal Name: Journal of Toxicology and Environmental Health Journal Issue: 4 Vol. 45; ISSN 0098-4108; ISSN JTEHD6
• Country of Publication: United States
• Language: English

Related Subjects

56 BIOLOGY AND MEDICINE
APPLIED STUDIES
BENZENE
BIOCHEMISTRY
BIOLOGICAL EFFECTS
CARCINOGENESIS
LIVER
RATS