Differential uptake of liposomes varying in size and lipid composition by parenchymal and Kupffer cells of mouse liver
Using liposomes differing in size and lipid composition, we have studied the uptake characteristics of the liver parenchymal and Kupffer cells. Desferal labeled with iron-59 was chosen as a radiomarker for the liposomal content, because Desferal in its free form does not cross cellular membranes. At various time intervals after an intravenous injection of liposomes into mice, the liver was perfused with collagenase, and the cells were separated in a Percoll gradient. It was found that large multilamellar liposomes (diameter of about 0.5 ..mu..m) were mainly taken up by the Kupffer cells. For these large liposomes, the rate of uptake by Kupffer cells was rapid, with maximum uptake at around 2 hours after liposome injection. In contrast, parenchymal cells were more effective in taking up small liposomes and the uptake of large liposomes was negligible. In addition, liposomes made with a galactolipid as part of the lipid constituents appeared to have higher affinity to parenchymal cells than liposomes made without the galactolipid. These findings should be of importance in designing suitable liposomes for drug targeting.
- Research Organization:
- Argonne National Lab., IL
- DOE Contract Number:
- W-31109-ENG-38
- OSTI ID:
- 6326113
- Journal Information:
- Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 31:19; ISSN LIFSA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CELL CONSTITUENTS
CELL MEMBRANES
CHEMICAL COMPOSITION
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
EVEN-ODD NUCLEI
GLANDS
INTERMEDIATE MASS NUCLEI
IRON 59
IRON ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIPIDS
LIPOSOMES
LIVER
MAMMALS
MEMBRANE TRANSPORT
MEMBRANES
METABOLISM
MICE
NUCLEI
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
PHYSIOLOGY
RADIOISOTOPES
RODENTS
TRACER TECHNIQUES
UPTAKE
VERTEBRATES