Influence of copper deficiency on binding and uptake of apolipoprotein E-free high density lipoproteins (APO E-free HDL) by isolated rat liver parenchymal and Kupffer cells

Journal Article · Wed Feb 01 04:00:00 EST 1989 · FASEB Journal (Federation of American Societies for Experimental Biology); (USA)

OSTI ID:6831752

Lei, K Y; Hendriks, H F.J.; Brouwer, A; Bock, I; De Ruiter, C S.J.; Knook, D L ^[1]

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1. Univ. of Arizona, Tucson (USA)

Weanling male Sprague-Dawley rats were assigned to two dietary treatments; copper-deficient (CD, 0.7 ppm) and adequate (CA, 7.0 ppm). After 8 weeks, plasma apo E-free HDL were isolated by a combination of ultracentrifugation, gel filtration, and heparin-Sepharose affinity chromatography. Liver parenchymal (P) and Kupffer (K) cells were obtained by collagenase perfusion and purified by elutriation. Freshly isolated cells were then incubated with {sup 125}I-apo E-free HDL, either from the same treatment group or in a crossover design, in order to establish if treatment differences were associated with cells and/or lipoproteins. Binding studies performed at 4{degree}C with increasing apo E-free HDL concentration indicated an increase in specific binding in P cells from CD rats. In cell association studies at 37{degree}C, P cells from CD rats demonstrated increased in trypsin releasable (surface-bound) and total cell-associated apo E-free HDL. In contrast, K cells from CD rats demonstrated a reduction in apo E-free HDL uptake (internalized). These findings confirm data obtained from cultured P cells and indicated that copper deficiency may exert different effects on lipoprotein metabolism in rat liver parenchymal and Kupffer cells.

• OSTI ID: 6831752
• Journal Information: FASEB Journal (Federation of American Societies for Experimental Biology); (USA), Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (USA) Vol. 3:4; ISSN FAJOE; ISSN 0892-6638
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
APOLIPOPROTEINS
BETA DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
COPPER
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTS
HALOGEN COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE COMPOUNDS
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIPIDS
LIPOPROTEINS
LIVER CELLS
MAMMALS
METABOLISM
METALS
NUCLEI
NUTRITIONAL DEFICIENCY
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIOISOTOPES
RATS
RODENTS
SOMATIC CELLS
TRACER TECHNIQUES
TRANSITION ELEMENTS
VERTEBRATES