Differential uptake of liposomes varying in size and lipid composition by parenchymal and Kupffer cells of mouse liver

Journal Article · Mon Nov 08 04:00:00 EST 1982 · Life Sci.; (United States)

DOI:https://doi.org/10.1016/0024-3205(82)90098-4· OSTI ID:6123649

Rahman, Y E; Cerny, E A; Patel, K R; Lau, E H; Wright, B J

Using liposomes differing in size and lipid composition, we have studied the uptake characteristics of the liver parenchymal and Kupffer cells. Desferal labeled with iron-59 was chosen as a radiomarker for the liposomal content, because Desferal in its free form does not cross cellular membranes.At various time intervals after an intravenous injection of liposomes into mice, the liver was perfused with collagenase, and the cells were separated in a Percoll gradient. It was found that large multilamellar liposomes (diameter of about 0.5 ..mu..m) were mainly taken up by the Kupffer cells. For these large liposomes, the rate of uptake by Kupffer cells was rapid, with maximum uptake at around 2 hours after liposme injection. Unexpectedly, small unilamellar liposomes (diameter of about 0.08 ..mu..m) were less effectively taken up by Kupffer cells, and the rate of uptake was slow, with a maximum uptake at about 10 hours after liposome injection. In contrast, parenchymal cells were more effective in taking up small liposmes and the uptake of large liposomes was negligible. In addition, liposomes made with a galactolipid as part of the lipid constituents appeared to have higher affinity to parenchymal cells than liposomes made without the galactolipid. These findings should be of importance in designing suitable liposomes for drug targeting.

• DOE Contract Number: W-31109-ENG-38
• OSTI ID: 6123649
• Journal Information: Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 31:19; ISSN LIFSA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BODY
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
EVEN-ODD NUCLEI
GLANDS
INJECTION
INTAKE
INTERMEDIATE MASS NUCLEI
INTRAVENOUS INJECTION
IRON 59
IRON ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LIPIDS
LIPOSOMES
LIVER
LIVER CELLS
MAMMALS
MICE
NUCLEI
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
PARTICLE SIZE
PHAGOCYTOSIS
RADIOISOTOPES
REACTION KINETICS
RETICULOENDOTHELIAL SYSTEM
RODENTS
SIZE
SOMATIC CELLS
TISSUES
TRACER TECHNIQUES
UPTAKE
VERTEBRATES