Genetic analysis of suppressors of the PF10 mutation in Chlamydomonas reinhardtii

Journal Article · Thu Dec 01 04:00:00 EST 1988 · Genetics; (United States)

OSTI ID:6324606

Dutcher, S K; Gibbons, W; Inwood, W B

A mutation at the PF10 locus of the unicellular green alga Chlamydomonas reinhardtii leads to abnormal cell motility. The asymmetric form of the ciliary beat stroke characteristic of wild-type flagella is modified by this mutation to a nearly symmetric beat. We report here that this abnormal motility is a conditional phenotype that depends on light intensity. In the absence of light or under low light intensities, the motility is more severely impaired than at higher light intensities. By UV mutagenesis we obtained 11 intragenic and 70 extragenic strains that show reversion of the pf10 motility phenotype observed in low light. The intragenic events reverted the motility phenotype of the pf10 mutation completely. The extragenic events define at least seven suppressor loci; these map to linkage groups IV, VII, IX, XI, XII and XVII. Suppressor mutations at two of the seven loci (LIS1 and LIS2) require light for their suppressor activity. Forty-eight of the 70 extragenic suppressors were examined in heterozygous diploid cells; 47 of these mutants were recessive to the wild-type allele and one mutant (bop5-1) was dominant to the wild-type allele. Complementation analysis of the 47 recessive mutants showed unusual patterns. Most mutants within a recombinationally defined group failed to complement one another, although there were pairs that showed intra-allelic complementation. Additionally, some of the mutants at each recombinationally defined locus failed to complement mutants at other loci. They define dominant enhancers of one another.

• Research Organization: Univ. of Colorado, Boulder (USA)
• OSTI ID: 6324606
• Journal Information: Genetics; (United States), Journal Name: Genetics; (United States) Vol. 120:4; ISSN GENTA
• Country of Publication: United States
• Language: English

Related Subjects

560130* -- Radiation Effects on Microorganisms
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALGAE
BEHAVIOR
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CHLAMYDOMONAS
CHLOROPHYCOTA
ELECTROMAGNETIC RADIATION
GENE RECOMBINATION
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
MICROORGANISMS
MUTATIONS
PHENOTYPE
PLANTS
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
ULTRAVIOLET RADIATION
UNICELLULAR ALGAE