Suppressors of a Saccharomyces cerevisiae pkc1 mutation identify alleles of the phosphatase gene PTC1 and of a novel gene encoding a putative basic leucine zipper protein

Journal Article · Fri Dec 01 04:00:00 EST 1995 · Genetics

OSTI ID:535341

Huang, K N; Symington, L S ^[1]

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1. Columbia Univ. College of Physicians and Surgeons, New York, NY (United States)

The PKC1 gene product, protein kinase C, regulates a mitogen-activated protein kinase (MAPK) cascade, which is implicated in cell wall metabolism. Previously, we identified the pkc1-4 allele in a screen for mutants with increased rates of recombination, indicating that PKC1 may also regulate DNA metabolism. The pkc1-4 allele also conferred a temperature-sensitive (ts) growth defect. Extragenic suppressors were isolated that suppress both the ts and hyperrecombination phenotypes conferred by the pkc1-4 mutation. Eight of these suppressors fell into two complementation groups, designated KCS1 and KCS2. KCS1 was cloned and found to encode a novel protein with homology to the basic leucine zipper family of transcription factors. KCS2 is allelic with PTC1, a previously identified type 2C serine/threonine protein phosphatase. Although mutation of either KCS1 or PTC1 causes little apparent phenotype, the kcs1{Delta} ptc1{Delta} double mutant fails to grow at 30{degrees}. Furthermore, the ptc1 deletion mutation is synthetically lethal in combination with a mutation in MPK1, which encodes a MAPK homologue proposed to act in the PKC1 pathway. Because PTC1 was initially isolated as a component of the Hog1p MAPK pathway, it appears that these two MAPK cascades share a common regulatory feature. 36 refs., 11 figs., 2 tabs.

• OSTI ID: 535341
• Journal Information: Genetics, Journal Name: Genetics Journal Issue: 4 Vol. 141; ISSN GENTAE; ISSN 0016-6731
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
AMINO ACID SEQUENCE
CELL WALL
DNA
DNA SEQUENCING
DNA-CLONING
GENE MUTATIONS
GENE RECOMBINATION
GENE REGULATION
GROWTH
LETHAL MUTATIONS
LEUCINE
METABOLISM
MITOGENS
MUTANTS
PHENOTYPE
PHOSPHATASES
PROTEINS
SACCHAROMYCES CEREVISIAE
SERINE
TEMPERATURE DEPENDENCE
THREONINE
TRANSCRIPTION
TRANSCRIPTION FACTORS