Mutagenic effect of tritium on DNA of Drosophila melanogaster: Technical progress report, December 15, 1986-July 15, 1987

Technical Report · Thu Jan 01 04:00:00 EST 1987

OSTI ID:6170408

Lee, W R

Recombinant DNA techniques were used to analyze mutants induced by either tritium or x-ray. Mutations induced at the alcohol dehydrogenase locus (Adh) in Drosophila melanogaster were first characterized by genetic complementation tests to determine if a multi-locus deletion has occurred. Mutants that are intragenic as defined by the complementation test are then placed opposite a deficiency so that the DNA from the mutant allele may be extracted and analyzed. Part I of the project is to analyze mutants induced by ionizing radiation with molecular techniques, and part II is to determine the molecular effects of these mutant phenotypes on their expression in the polypeptide produced by the mutant gene. Part III of this project consists of inducing mutants with tritiated water at the Adh locus in D. melanogaster. We have reported the development of a feeding method for exposing male D. melanogaster to tritiated water that would give a range in dose from 6.66 Gy to 26.64 Gy. This method of exposing Drosophila was used first to study a dose response curve for tritium using as a genetic endpoint the sex-linked recessive lethal test. 3 figs., 1 tab.

• Research Organization: Louisiana State Univ., Baton Rouge (USA). Dept. of Zoology and Physiology
• DOE Contract Number: FG05-86ER60393
• OSTI ID: 6170408
• Report Number(s): DOE/ER/60393-2; ON: DE87013456
• Country of Publication: United States
• Language: English

Related Subjects

550400 -- Genetics
560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
ARTHROPODS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
DIPTERA
DOSE-RESPONSE RELATIONSHIPS
DROSOPHILA
ELECTROMAGNETIC RADIATION
ENZYMES
FLIES
FRUIT FLIES
GENE MUTATIONS
HYDROGEN ISOTOPES
INSECTS
INVERTEBRATES
IONIZING RADIATIONS
ISOTOPES
LETHAL MUTATIONS
LIGHT NUCLEI
MUTAGENESIS
MUTATIONS
NUCLEI
ODD-EVEN NUCLEI
OXIDOREDUCTASES
RADIATIONS
RADIOISOTOPES
TRITIUM
X RADIATION
YEARS LIVING RADIOISOTOPES