Molecular analysis of mutant and wild type alcohol dehydrogenase alleles from Drosophila
Thesis/Dissertation
·
OSTI ID:7098331
Wild type alcohol dehydrogenase polypeptides (ADH) from Drosophila melanogaster transformants were examined using western blots and polyclonal antiserum specific for Drosophila melanogaster ADH. Mutants induced in Drosophila spermatozoa at the alcohol dehydrogenase (Adh) locus using X-rays, 1-ethyl-1-nitrosourea (ENU) or ethyl methanesulfonate (EMS) were characterized using genetic complementation tests, western blots, Southern blots, northern blots and enzymatic amplification of the Adh locus. Genetic complementation tests showed that 22/30 X-ray-induced mutants, and 3/13 ENU and EMS induced mutants were multi-locus deficiencies. Western blot analysis of the intragenic mutations showed that 4/7 X-ray-induced mutants produced detectable polypeptides, one of which was normal in molecular weight and charge. In contrast 8/10 intragenic ENU and EMS induced mutants produced normal polypeptides. Southern blot analysis showed that 5/7 intragenic X-ray induced mutants and all 10 of the intragenic ENU and EMS induced mutants were normal with respect to the alleles they were derived from.
- Research Organization:
- Louisiana State Univ. and Agricultural and Mechanical Coll., Baton Rouge, LA (USA)
- OSTI ID:
- 7098331
- Country of Publication:
- United States
- Language:
- English
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· Environmental and Molecular Mutagenesis; (United States)
·
OSTI ID:6527918
Related Subjects
560152 -- Radiation Effects on Animals-- Animals
560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALCOHOL DEHYDROGENASE
ANIMALS
ARTHROPODS
BIOLOGICAL EFFECTS
DIPTERA
DROSOPHILA
ELECTROMAGNETIC RADIATION
EMS
ENZYMES
ESTERS
FLIES
FRUIT FLIES
GAMETES
GENE AMPLIFICATION
GENE MUTATIONS
GENETIC EFFECTS
GERM CELLS
HEMIACETAL DEHYDROGENASES
INSECTS
INVERTEBRATES
IONIZING RADIATIONS
MUTAGENS
MUTANTS
MUTATIONS
NITROSO COMPOUNDS
NITROSOUREAS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OXIDOREDUCTASES
RADIATION INDUCED MUTANTS
RADIATIONS
SPERMATOZOA
SULFONIC ACID ESTERS
X RADIATION
560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALCOHOL DEHYDROGENASE
ANIMALS
ARTHROPODS
BIOLOGICAL EFFECTS
DIPTERA
DROSOPHILA
ELECTROMAGNETIC RADIATION
EMS
ENZYMES
ESTERS
FLIES
FRUIT FLIES
GAMETES
GENE AMPLIFICATION
GENE MUTATIONS
GENETIC EFFECTS
GERM CELLS
HEMIACETAL DEHYDROGENASES
INSECTS
INVERTEBRATES
IONIZING RADIATIONS
MUTAGENS
MUTANTS
MUTATIONS
NITROSO COMPOUNDS
NITROSOUREAS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OXIDOREDUCTASES
RADIATION INDUCED MUTANTS
RADIATIONS
SPERMATOZOA
SULFONIC ACID ESTERS
X RADIATION