(Mutagenic effect of tritium on DNA of Drosophila melanogaster)

Technical Report · Tue Jan 01 04:00:00 EST 1991

OSTI ID:5760856

A series of Adh mutants induced in Drosophila by X-rays were compared to Adh mutants induced by tritiated water. Sequence analysis of 8 intragenic null mutations suggest a double strand break might occur, consistent with our linear dose response curve. Additional data are required before we accept this model; it is hoped experiments with {sup 60}Co will yield more intragenic deletions. Our current research project involves genetic and molecular analysis of {sup 3}H beta induced Adh null mutations. All 23 mutations induced at the Adh locus have been multilocus deletions. This is in contrast to results seen with X-ray induction of mutation; as a consequence, the emphasis of our molecular analysis has switched from sequencing intragenic mutations to developing methods for sequencing the break points of deletions. This will permit us to determine if our model of deletion formation is applicable outside the structural gene. 13 refs., 8 figs., 5 tabs.

• Research Organization: Louisiana State Univ., Baton Rouge, LA (USA)
• Sponsoring Organization: DOE; USDOE, Washington, DC (USA)
• DOE Contract Number: FG05-86ER60393
• OSTI ID: 5760856
• Report Number(s): DOE/ER/60393-6; ON: DE91014921
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALCOHOL DEHYDROGENASE
ANIMALS
ARTHROPODS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CHROMOSOMAL ABERRATIONS
CHROMOSOME BREAKAGE
COBALT 60
COBALT ISOTOPES
DIPTERA
DNA
DNA HYBRIDIZATION
DNA REPAIR
DNA SEQUENCING
DOCUMENT TYPES
DOSE-RESPONSE RELATIONSHIPS
DROSOPHILA
ELECTROMAGNETIC RADIATION
ENZYMES
FLIES
FRUIT FLIES
GAMETES
GENE MUTATIONS
GENETIC MAPPING
GERM CELLS
HEMIACETAL DEHYDROGENASES
HYBRIDIZATION
HYDROGEN ISOTOPES
INSECTS
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
INVERTEBRATES
IONIZING RADIATIONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LETHAL MUTATIONS
LIGHT NUCLEI
MAPPING
MINUTES LIVING RADIOISOTOPES
MUTAGENESIS
MUTATION FREQUENCY
MUTATIONS
NUCLEI
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PROGRESS REPORT
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RBE
RECOMBINANT DNA
RECOVERY
REPAIR
SPERMATOZOA
STRAND BREAKS
STRUCTURAL CHEMICAL ANALYSIS
TRITIUM
X RADIATION
YEARS LIVING RADIOISOT
YEARS LIVING RADIOISOTOPES