Mutagenic effect of tritium on DNA of Drosophila melanogaster: Comprehensive performance report, June 1, 1988--June 30, 1989

Technical Report · Sun Jan 01 04:00:00 EST 1989

DOI:https://doi.org/10.2172/5893552· OSTI ID:5893552

Lee, W. R.

The first objective was to induce mutations at the Adh locus in Drosophila melanogaster using the procedures and dosimetry that we have reprints developed. The linear dose response for the doses used by others would indicate that at that high dose, the mutations will be based predominately on the same mechanisms of mutagenesis as that of lower exposure levels. The frequency of mutations induced at 25G is 100 times the spontaneous frequency; therefore, the mutation spectrum should be that of the tritium beta radiation with a non significant contribution from spontaneous mutations. These tritium induced null mutations were then analyzed by complementation genetic tests using both deletions and specific locus mutations near the Adh locus. Following the genetic analysis, molecular analysis will be conducted on these mutations. 1 tab.

• Research Organization: Louisiana State Univ., Baton Rouge, LA (USA). Dept. of Zoology and Physiology
• DOE Contract Number: FG05-86ER60393
• OSTI ID: 5893552
• Report Number(s): DOE/ER/60393-4; ON: DE89015925
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
ARTHROPODS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
DIPTERA
DOCUMENT TYPES
DOSIMETRY
DROSOPHILA
FLIES
FRUIT FLIES
HYDROGEN ISOTOPES
INSECTS
INVERTEBRATES
ISOTOPES
LIGHT NUCLEI
MUTATION FREQUENCY
MUTATIONS
NUCLEI
ODD-EVEN NUCLEI
PROGRESS REPORT
RADIOINDUCTION
RADIOISOTOPES
RBE
STRAND BREAKS
TRITIUM
YEARS LIVING RADIOISOTOPES