DNA sequence analysis of X-ray induced Adh null mutations in Drosophila melanogaster

Journal Article · Tue Jan 01 04:00:00 EST 1991 · Environmental and Molecular Mutagenesis; (United States)

DOI:https://doi.org/10.1002/em.2850180303· OSTI ID:6094696

Mahmoud, J; Fossett, N G; Arbour-Reily, P; McDaniel, M; Tucker, A; Chang, S H; Lee, W R ^[1]

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1. Louisiana State Univ., Baton Rouge (United States)

The mutational spectrum for 28 X-ray induced mutations and 2 spontaneous mutations, previously determined by genetic and cytogenetic methods, consisted of 20 multilocus deficiencies (19 induced and 1 spontaneous) and 10 intragenic mutations (9 induced and 1 spontaneous). One of the X-ray induced intragenic mutations was lost, and another was determined to be a recombinant with the allele used in the recovery scheme. The DNA sequence of two X-ray induced intragenic mutations has been published. This paper reports the results of DNA sequence analysis of the remaining intragenic mutations and a summary of the X-ray induced mutational spectrum. The combination of DNA sequence analysis with genetic complementation analysis shows a continuous distribution in size of deletions rather than two different types of mutations consisting of deletions and point mutations'. Sequencing is shown to be essential for detecting intragenic deletions. Of particular importance for future studies is the observation that all of the intragenic deletions consist of a direct repeat adjacent to the breakpoint with one of the repeats deleted.

• DOE Contract Number: FG05-86ER60393
• OSTI ID: 6094696
• Journal Information: Environmental and Molecular Mutagenesis; (United States), Journal Name: Environmental and Molecular Mutagenesis; (United States) Vol. 18:3; ISSN 0893-6692; ISSN EMMUE
• Country of Publication: United States
• Language: English

Related Subjects

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560300 -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALCOHOL DEHYDROGENASE
ANIMALS
ARTHROPODS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
DIPTERA
DNA SEQUENCING
DROSOPHILA
ELECTROMAGNETIC RADIATION
ENZYMES
FLIES
FRUIT FLIES
GENE MUTATIONS
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
HEMIACETAL DEHYDROGENASES
INSECTS
INVERTEBRATES
IONIZING RADIATIONS
MUTATIONS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PROTEINS
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
SPONTANEOUS MUTATIONS
STRUCTURAL CHEMICAL ANALYSIS
X RADIATION