Addition of molecular methods to mutation studies with Drosophila melanogaster

Journal Article · Sun Jan 01 04:00:00 EST 1989 · Environmental and Molecular Mutagenesis; (USA)

DOI:https://doi.org/10.1002/em.2850140618· OSTI ID:7006975

Lee, W R ^[1]

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1. Louisiana State Univ., Baton Rouge, LA (USA)

For 80 years, Drosophila melanogaster has been used as a major tool in analyzing Mendelian genetics. By using chromosome inversions that suppress crossing over, geneticists have developed a large number of stocks for mutation analysis. These stocks permit numerous tests for specific locus mutations, lethals at multiple loci on any chromosome, chromosome exchanges, insertions, and deletions. The entire genome can be manipulated for a degree of genetic control not found in other germ-line systems. Recombinant DNA techniques now permit analysis of mutations to the nucleotide level. By combining classical genetic analysis with recombinant DNA techniques, it is possible to analyze mutations that range from chromosome aberrations and multilocus deficiencies to single nucleotide transitions.

• DOE Contract Number: FG05-86ER60393
• OSTI ID: 7006975
• Journal Information: Environmental and Molecular Mutagenesis; (USA), Journal Name: Environmental and Molecular Mutagenesis; (USA) Vol. 14:16; ISSN EMMUE; ISSN 0893-6692
• Country of Publication: United States
• Language: English

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