Conditioned medium from irradiated bovine pulmonary artery endothelial cells stimulates increased protein synthesis by irradiated bovine lung fibroblasts in vitro

Journal Article · Sat Sep 01 04:00:00 EDT 1990 · Experimental Lung Research; (USA)

DOI:https://doi.org/10.3109/01902149009068817· OSTI ID:6320960

Flavin, M P; Parton, L A; Bowman, C M ^[1]

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1. Childrens Hospital of Los Angeles, CA (USA)

Pulmonary fibrosis, a potentially fatal consequence of radiation exposure, occurs by unknown mechanisms. The hypothesis that endothelial cells, injured by radiation, could alter the biochemical function of lung fibroblasts, was tested by exposing cultures of bovine pulmonary artery endothelial cells to 0 or 5 Gy radiation and then incubating them in fresh medium for 48 h. This endothelial cell conditioned medium (ECCM) was then applied to irradiated or nonirradiated cultures of bovine lung fibroblasts. Forty-eight hours later the fibroblasts were analyzed for their ability to synthesize DNA and protein. The ECCM from injured cells stimulated fibroblast protein synthesis twofold to threefold in irradiated fibroblasts without increasing DNA synthesis. It also stimulated a significant but less marked increase in protein synthesis in nonirradiated fibroblasts. Two-dimensional gel electrophoresis revealed this increased synthesis to be expressed in less than 10% of the 1100 separable fibroblast proteins. This study shows that endothelial cells injured by radiation produce factors that stimulate injured fibroblasts to markedly increase their synthesis of certain intracellular proteins, while not stimulating fibroblast replication.

• OSTI ID: 6320960
• Journal Information: Experimental Lung Research; (USA), Journal Name: Experimental Lung Research; (USA) Vol. 16:5; ISSN 0190-2148; ISSN EXLRD
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
AMINO ACIDS
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
ARTERIES
AZOLES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOSYNTHESIS
BLOOD VESSELS
BODY
CARBOXYLIC ACIDS
CARDIOVASCULAR SYSTEM
CATTLE
CONNECTIVE TISSUE CELLS
CULTURE MEDIA
DISEASES
DNA REPLICATION
DOMESTIC ANIMALS
ELECTROPHORESIS
ENDOTHELIUM
FIBROBLASTS
FIBROSIS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
LEUCINE
LUNGS
MAMMALS
NUCLEIC ACID REPLICATION
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PATHOLOGICAL CHANGES
PROLINE
PROTEINS
PYRROLES
PYRROLIDINES
RADIATION EFFECTS
RADIOINDUCTION
RESPIRATORY SYSTEM
RESPIRATORY SYSTEM DISEASES
RUMINANTS
SOMATIC CELLS
SYNTHESIS
TISSUES
TWO-DIMENSIONAL ELECTROPHORESIS
VERTEBRATES