Endothelial injury and repair in radiation-induced pulmonary fibrosis

Journal Article · Mon Aug 01 04:00:00 EDT 1983 · Am. J. Pathol.; (United States)

OSTI ID:5545354

Adamson, I Y; Bowden, D H

Cytokinetic relationships between endothelial cells and fibroblasts during lung injury and repair in mice have been studied in a morphologic, autoradiographic, and biochemical study following whole body irradiation. After 650 rads, endothelial injury accompanied by interstitial edema was seen between weeks 1 and 2. The cell labeling curve had two components: predominant endothelial labeling to 3 weeks, then a smaller rise in DNA synthesis in interstitial cells. There was focal fibrosis but little change in total hydroxyproline to 20 weeks. After 1000 rads, cell injury, still confined to the endothelium, was more severe and lasted up to 6 weeks. Increased DNA synthesis occurred in the endothelium between Weeks 2 and 8 and in interstitial cells from Week 3 to 16, when total hydroxyproline was significantly elevated and many fibrotic areas were seen in the lung. The results indicate that acute endothelial injury may be rapidly repaired with little fibroblastic stimulation, whereas severe or prolonged injury with delayed regeneration disturbs endothelial-mesenchymal relationships. This may be a key factor in promoting fibroblast proliferation and the deposition of collagen.

• Research Organization: Pathology Department, University of Manitoba, Winnipeg, Canada
• OSTI ID: 5545354
• Journal Information: Am. J. Pathol.; (United States), Journal Name: Am. J. Pathol.; (United States) Vol. 112:2; ISSN AJPAA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
AMINO ACIDS
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
AUTORADIOGRAPHY
AZOLES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BODY
CARBOXYLIC ACIDS
CELL DIFFERENTIATION
CHEMICAL ANALYSIS
CONNECTIVE TISSUE CELLS
DISEASES
DNA REPLICATION
DOSE-RESPONSE RELATIONSHIPS
EDEMA
ENDOTHELIUM
FIBROBLASTS
FIBROSIS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROXY ACIDS
HYDROXYPROLINE
INJURIES
LUNGS
MAMMALS
MICE
NUCLEIC ACID REPLICATION
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PATHOLOGICAL CHANGES
PYRROLES
PYRROLIDINES
QUANTITATIVE CHEMICAL ANALYSIS
RADIATION EFFECTS
RADIATION INJURIES
RECOVERY
REPAIR
RESPIRATORY SYSTEM
RESPIRATORY SYSTEM DISEASES
RODENTS
SOMATIC CELLS
SYMPTOMS
TISSUES
VERTEBRATES