Effects of ionizing radiation on the pulmonary endothelial cell uptake of alpha-aminoisobutyric acid and synthesis of prostacyclin

Journal Article · Thu May 01 04:00:00 EDT 1986 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3576791· OSTI ID:5651943

Friedman, M; Saunders, D S; Madden, M C; Chaney, E L; Kwock, L

The effects of gamma irradiation (150-3000 rad) on prostacyclin synthesis (PGI2) and Na+-dependent amino acid uptake (alpha-aminoisobutyric acid, AIB) were assessed in vitro in bovine pulmonary artery endothelial cells grown in plastic culture dishes. A dose-dependent increase in both PGI2 synthesis and AIB was found 24 h after irradiation at exposure levels greater than 600 rad. The increase in PGI2 synthesis (297% of sham-irradiated values at 3000 rad, P less than 0.01) was due to an increase in release of arachidonic acid from plasma membrane stores as well as stimulation of cyclooxygenase and/or prostacyclin synthetase enzymes. The increase in AIB uptake (75% increase at 3000 rad compared to sham-exposure values) correlated with the increased synthesis of PGI2 (r = 0.94). There was also a dose-dependent increase in the number of cells that became detached from the culture dishes during the 24-h period after irradiation. The changes in PGI2 synthesis and AIB uptake induced by gamma irradiation differed if the endothelial cells were grown on cover slips, indicating that the endothelial response to irradiation may be dependent on the interaction between the endothelial cell and its extracellular basement membrane matrix.

• Research Organization: Univ. of North Carolina, Chapel Hill
• OSTI ID: 5651943
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 2; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALI METALS
ANIMAL TISSUES
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOSYNTHESIS
BODY
CARBOXYLIC ACIDS
CATTLE
CELL CULTURES
DOMESTIC ANIMALS
DOSE-RESPONSE RELATIONSHIPS
DOSES
ELECTROMAGNETIC RADIATION
ELEMENTS
ENDOTHELIUM
GAMMA RADIATION
IONIZING RADIATIONS
ISOBUTYRIC ACID
LUNGS
MAMMALS
METALS
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PROSTAGLANDINS
RADIATION DOSES
RADIATION EFFECTS
RADIATIONS
RESPIRATORY SYSTEM
RUMINANTS
SODIUM
SYNTHESIS
TISSUES
UPTAKE
VERTEBRATES