Splenic thrombopoiesis after bone marrow ablation with radiostrontium: A murine model

Journal Article · Sat Dec 01 00:00:00 EST 1990 · Journal of Laboratory and Clinical Medicine; (USA)

OSTI ID:6319886

Davis, E; Corash, L; Baker, G; Mok, Y; Hill, R J; Levin, J ^[1]

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1. Univ. of California School of Medicine, San Francisco (USA)

Murine platelet production is normally supported by high-ploidy bone marrow megakaryocytes without significant contribution from splenic megakaryocytes with predominantly low-ploidy levels. We produced sustained bone marrow ablation using radiostrontium, and examined the processes by which splenic platelet production is initiated and maintained in the absence of bone marrow function. Bone marrow hematopoiesis, measured by total nucleated cell number and viability, megakaryocyte colony-forming cells, and granulocyte-macrophage colony-forming cells, was rapidly ablated in mice by using yttrium 90-free strontium 90. Platelet count declined from normal (1224 x 10(3)/microliters) to a nadir (98 x 10(3)/microliters) 11 days after 90Sr, and then rose to a stable level (705 x 10(3)/microliters) on days 20 through 115. Peripheral leukocyte concentration decreased rapidly and remained below 25% of normal in contrast to hemoglobin levels, which were minimally lowered. Mean spleen weight rose rapidly after 90Sr to 66% above normal. Splenic megakaryocyte frequency, measured by two-color fluorescence-activated flow cytometry, rose from basal levels (0.09% +/- 0.06%) to 0.15% +/- 0.07% (p less than 0.001), total spleen nucleated cells fell to 71% of normal, and the absolute number of spleen megakaryocytes was unchanged. Total spleen megakaryocyte colony-forming cells were not significantly increased above normal whereas total spleen granulocyte-macrophage colony-forming cells increased abruptly after day 13 to 10 times normal levels. Splenectomy after hematopoietic recovery from 90Sr bone marrow ablation resulted in a rapid decline of platelet levels, followed by death. Although the spleen became the sole site of platelet production, the splenic megakaryocyte ploidy distribution was only minimally changed from normal, and the modal ploidy class remained 2N.

• OSTI ID: 6319886
• Journal Information: Journal of Laboratory and Clinical Medicine; (USA), Vol. 116:6; ISSN 0022-2143
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BONE MARROW
BIOLOGICAL RADIATION EFFECTS
SPLEEN
THROMBOPOIESIS
BIOLOGICAL MODELS
BLOOD COUNT
BLOOD PLATELETS
CELL FLOW SYSTEMS
FLUORESCENCE
HEMATOPOIETIC SYSTEM
LEUKOCYTES
MICE
PLOIDY
SPLEEN COLONY FORMATION
STRONTIUM 90
TRACER TECHNIQUES
ALKALINE EARTH ISOTOPES
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD FORMATION
BODY
BODY FLUIDS
COLONY FORMATION
EVEN-EVEN NUCLEI
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LUMINESCENCE
MAMMALS
MATERIALS
NUCLEI
ORGANS
RADIATION EFFECTS
RADIOISOTOPES
RODENTS
STRONTIUM ISOTOPES
TISSUES
VERTEBRATES
YEARS LIVING RADIOISOTOPES
560162* - Radionuclide Effects
Kinetics
& Toxicology- Animals
Plants
Microorganisms
& Cells