Characteristics of rat megakaryocyte colonies and their progenitors in agar culture
Journal Article
·
· Exp. Hematol.; (United States)
OSTI ID:6255399
The characteristics of megakaryocyte colonies that develop from megakaryocyte progenitors of rat bone marrow stimulated by rat spleen-conditioned medium (SCM) in agar culture were investigated. Colony frequency was optimal on day 7 and increased relative to both the number of cells plated and the concentration of SCM used. Colonies were categorized as small cell and big cell. Small-cell colonies had a greater proliferative potential, with a mean of 25 cells/colony. Big-cell colonies averaged 15 cells/colony. The ratio of big-cell to small-cell colonies was 0.69 +/- 0.29. Granulocyte-macrophage colonies, which were also stimulated by SCM, accounted for 70% +/- 15% of the total colonies in the cultures. Cytocidal experiments with tritiated thymidine reduced megakaryocyte colony formation by 45% and granulocyte-macrophage colony formation by 21%. The properties of rat, mouse, and human megakaryocyte progenitors as assayed in vitro are compared.
- Research Organization:
- Centers for Disease Control, Atlanta, GA
- OSTI ID:
- 6255399
- Journal Information:
- Exp. Hematol.; (United States), Journal Name: Exp. Hematol.; (United States) Vol. 10; ISSN EXHMA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560122* -- Radiation Effects on Cells-- Internal Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AGAR
ANIMAL CELLS
ANIMALS
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BONE MARROW CELLS
CARBOHYDRATES
CELL KILLING
COLLOIDS
COLONY FORMATION
CONNECTIVE TISSUE CELLS
CULTURE MEDIA
DISPERSIONS
DNA REPLICATION
HETEROCYCLIC COMPOUNDS
KINETICS
LABELLED COMPOUNDS
MAMMALS
NUCLEIC ACID REPLICATION
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
POLYSACCHARIDES
PYRIMIDINES
RADIATION EFFECTS
RADIOINDUCTION
RATS
RIBOSIDES
RODENTS
SACCHARIDES
SOMATIC CELLS
THYMIDINE
TRITIUM COMPOUNDS
VERTEBRATES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AGAR
ANIMAL CELLS
ANIMALS
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BONE MARROW CELLS
CARBOHYDRATES
CELL KILLING
COLLOIDS
COLONY FORMATION
CONNECTIVE TISSUE CELLS
CULTURE MEDIA
DISPERSIONS
DNA REPLICATION
HETEROCYCLIC COMPOUNDS
KINETICS
LABELLED COMPOUNDS
MAMMALS
NUCLEIC ACID REPLICATION
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
POLYSACCHARIDES
PYRIMIDINES
RADIATION EFFECTS
RADIOINDUCTION
RATS
RIBOSIDES
RODENTS
SACCHARIDES
SOMATIC CELLS
THYMIDINE
TRITIUM COMPOUNDS
VERTEBRATES