Direct proliferative actions of stem cell factor on murine bone marrow cells in vitro: Effects of combination with colony-stimulating factors

Journal Article · Mon Jul 15 04:00:00 EDT 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.88.14.6239· OSTI ID:5451842

Metcalf, D; Nicola, N A ^[1]

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1. Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital Victoria (Australia)

Stem cell factor (SCF), the ligand for the c-kit protooncogene product, was able to stimulate blast cell and granulocytic colony formation by precursors from normal murine bone marrow. The blast cell colonies contained a high content of progenitor cells able to form macrophage and/or granulocyte colonies. Clone transfer studies, the secondary culture of colony cells, and the culture of populations freed of accessory cells all indicated a direct proliferative action of SCF. SCF receptors were present in high numbers on blast cells and in lower numbers on immature granulocytic, monocytic, and eosinophilic cells. Combination of SCF with granulocyte, granulocyte-macrophage, or multipotential colony-stimulating factors, but not macrophage colony-stimulating factor, resulted in enhancement of colony size. Granulocyte colony-stimulating factor enhanced cell proliferation initiated by SCF, but not vice-versa, and resulted in a 10-fold increase in colony cell numbers and a 7-fold increase in progenitor cells in blast colonies. No evidence was obtained that SCF, alone or in combination with granulocyte colony-stimulating factor, could stimulate self-generation by blast colony-forming cells.

• OSTI ID: 5451842
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (United States) Journal Issue: 14 Vol. 88:14; ISSN PNASA; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
AUTORADIOGRAPHY
BIOCHEMICAL REACTION KINETICS
BONE MARROW CELLS
CELL PROLIFERATION
COLONY FORMATION
CONNECTIVE TISSUE CELLS
GROWTH FACTORS
IODINE ISOTOPES
ISOTOPES
KINETICS
MACROPHAGES
MAMMALS
MICE
MITOGENS
ORGANIC COMPOUNDS
PHAGOCYTES
PROTEINS
REACTION KINETICS
RODENTS
SOMATIC CELLS
VERTEBRATES