Isolation of a murine osteoclast colony-stimulating factor

Journal Article · Tue Oct 01 04:00:00 EDT 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.88.19.8500· OSTI ID:5826717

Lee, M Y; Eyre, D R; Osborne, W R.A. ^[1]

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1. Univ. of Washington, Seattle (United States)

Cultures of a cell line derived from a murine mammary carcinoma that induces hypercalcemia were examined for soluble products that could induce osteoclasts to differentiate from murine bone marrow cells. The serum-free culture supernatant of this cell line stimulated growth of colonies from bone marrow cells that exhibited tartrate-resistant acid phosphatase (TRAPase) activity. These TRAPase-positive cells demonstrated essential features of osteoclasts when cocultured with mineralized bone or dentin. The culture period required for colony development and the frequency of colony-forming cells indicated that relatively primitive marrow progenitors were stimulated by a tumor-derived factor(s) to form immature osteoclasts. Other colony-stimulating factors (CSFs), including granulocyte CSF, macrophage CSF, granulocyte-macrophase CSF and interleukin 3, were ruled out as the source of the activity produced by the tumor cells. The biological activity was successfully purified by gel filtration chromatography and reverse-phase HPLC. By SDS/PAGE, the activity was traced to a protein of {approx}17 kDa. Functional and biochemical studies of the purified factor suggest that it is distinct from any known CSF of myeloid cells. This protein appears to be a CSF for the osteoclast lineage, osteoclast CSF (O-CSF).

• DOE Contract Number: FG06-86ER60409
• OSTI ID: 5826717
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (United States) Vol. 88:19; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ACID PHOSPHATASE
ANIMAL CELLS
BODY
BONE CELLS
BONE MARROW CELLS
CARBOXYLIC ACID SALTS
CELL DIFFERENTIATION
CHROMATOGRAPHY
CONNECTIVE TISSUE CELLS
ELECTRON MICROSCOPY
ELECTROPHORESIS
ENZYME ACTIVITY
ENZYMES
ESTERASES
GLANDS
GROWTH FACTORS
HYDROLASES
LIQUID COLUMN CHROMATOGRAPHY
MACROPHAGES
MAMMARY GLANDS
MICROSCOPY
MITOGENS
MOLECULAR STRUCTURE
ORGANIC COMPOUNDS
ORGANS
PHAGOCYTES
PHOSPHATASES
PROTEINS
SCANNING ELECTRON MICROSCOPY
SEPARATION PROCESSES
SOMATIC CELLS
TARTRATES
TUMOR CELLS