Effects of recombinant granulocyte colony-stimulating factor (rG-CSF) and recombinant granulocyte-macrophage colony-stimulating factor (rGM-CSF) on acute radiation hematopoietic injury in mice

Journal Article · Fri Sep 01 00:00:00 EDT 1989 · Experimental Hematology; (USA)

OSTI ID:5466573

Tanikawa, S; Nakao, I; Tsuneoka, K; Nara, N ^[1]

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1. National Institute of Radiological Sciences, Chiba (Japan)

We have attempted to evaluate in vivo effects of granulocyte colony-stimulating factor (G-CSF) and granulocyte-macrophage colony-stimulating factor (GM-CSF) on acute radiation hematopoietic injury in mice. BDF1 mice, irradiated with 7.5-Gy x-rays, were injected i.p. twice daily for 10 days with 10(5) U recombinant human G-CSF, 3.75 x 10(5) U recombinant murine GM-CSF, or a combination of both. G-CSF significantly enhanced the recovery of not only peripheral leukocytes but also platelets and hematocrit on days 14 and 21 after irradiation. GM-CSF significantly enhanced the recovery of platelets on day 14 and peripheral leukocytes on day 21. G-CSF markedly enhanced the recovery of spleen colony-forming units (CFU-S), colony-forming units in culture (CFU-C), erythroid burst-forming units (BFU-E), and megakaryocyte colony-forming units (CFU-Meg) both in bone marrow and in the spleen. GM-CSF significantly enhanced the recovery of CFU-Meg in bone marrow on day 14. We found synergistic effects between G-CSF and GM-CSF on CFU-S, CFU-C, and CFU-Meg in the spleen on day 14, although we found antagonistic effects between G-CSF and GM-CSF on CFU-S and CFU-C in bone marrow on day 7, and on platelet counts on day 7. These results indicate that the administration of recombinant G-CSF and GM-CSF may be useful in accelerating hematopoietic recovery in patients with acute radiation hematopoietic injuries.

• OSTI ID: 5466573
• Journal Information: Experimental Hematology; (USA), Vol. 17:8; ISSN 0301-472X
• Country of Publication: United States
• Language: English

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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
GROWTH FACTORS
RADIOSENSITIVITY EFFECTS
HEMATOPOIETIC SYSTEM
BIOLOGICAL RADIATION EFFECTS
ACUTE IRRADIATION
BIOLOGICAL RECOVERY
BLOOD COUNT
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BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CELL CONSTITUENTS
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
INJURIES
IONIZING RADIATIONS
IRRADIATION
MAMMALS
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560152* - Radiation Effects on Animals- Animals