Effects of recombinant granulocyte colony-stimulating factor (rG-CSF) and recombinant granulocyte-macrophage colony-stimulating factor (rGM-CSF) on acute radiation hematopoietic injury in mice

Journal Article · Fri Sep 01 00:00:00 EDT 1989 · Experimental Hematology; (USA)

OSTI ID:5466573

Tanikawa, S; Nakao, I; Tsuneoka, K; Nara, N ^[1]

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1. National Institute of Radiological Sciences, Chiba (Japan)

We have attempted to evaluate in vivo effects of granulocyte colony-stimulating factor (G-CSF) and granulocyte-macrophage colony-stimulating factor (GM-CSF) on acute radiation hematopoietic injury in mice. BDF1 mice, irradiated with 7.5-Gy x-rays, were injected i.p. twice daily for 10 days with 10(5) U recombinant human G-CSF, 3.75 x 10(5) U recombinant murine GM-CSF, or a combination of both. G-CSF significantly enhanced the recovery of not only peripheral leukocytes but also platelets and hematocrit on days 14 and 21 after irradiation. GM-CSF significantly enhanced the recovery of platelets on day 14 and peripheral leukocytes on day 21. G-CSF markedly enhanced the recovery of spleen colony-forming units (CFU-S), colony-forming units in culture (CFU-C), erythroid burst-forming units (BFU-E), and megakaryocyte colony-forming units (CFU-Meg) both in bone marrow and in the spleen. GM-CSF significantly enhanced the recovery of CFU-Meg in bone marrow on day 14. We found synergistic effects between G-CSF and GM-CSF on CFU-S, CFU-C, and CFU-Meg in the spleen on day 14, although we found antagonistic effects between G-CSF and GM-CSF on CFU-S and CFU-C in bone marrow on day 7, and on platelet counts on day 7. These results indicate that the administration of recombinant G-CSF and GM-CSF may be useful in accelerating hematopoietic recovery in patients with acute radiation hematopoietic injuries.

• OSTI ID: 5466573
• Journal Information: Experimental Hematology; (USA), Journal Name: Experimental Hematology; (USA) Vol. 17:8; ISSN EXHMA; ISSN 0301-472X
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACUTE EXPOSURE
ACUTE IRRADIATION
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BLOOD
BLOOD CELLS
BLOOD COUNT
BODY
BODY FLUIDS
BONE MARROW CELLS
CELL CONSTITUENTS
CELL NUCLEI
COLONY FORMATION
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
FEMUR
GROWTH FACTORS
HEMATOPOIETIC SYSTEM
INJURIES
IONIZING RADIATIONS
IRRADIATION
LEUKOCYTES
MAMMALS
MATERIALS
MICE
MITOGENS
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIATION EFFECTS
RADIATION INJURIES
RADIATIONS
RADIOSENSITIVITY EFFECTS
RECOVERY
RODENTS
SKELETON
SOMATIC CELLS
SPLEEN
STEM CELLS
TIBIA
VERTEBRATES
X RADIATION