Inhibition of growth of normal murine granulocytes by cocultured acute leukemic cells

Journal Article · Tue Nov 01 04:00:00 EST 1977 · Blood; (United States)

OSTI ID:7208168

Miller, A M; Page, P L; Hartwell, B L; Robinson, S H

Coculture of equal numbers of C1498 murine acute myelogenous leukemia cells and normal mouse bone marrow cells in diffusion chambers (DC) led to complete suppression of normal granulocyte development within a few days. Suppression of granulopoiesis was not observed with irradiation-killed leukemic cells. Direct interaction between the leukemic and normal cells appears to be necessary since there was no inhibition when the two populations were separated by an intervening Millipore filter in double-DC cultures. When the ratio of leukemic to normal cells was reduced from 50/50 to 15/85, proliferation of granulocytic cells remained normal for a longer time but eventually ceased. With both the 15/85 and 50/50 cultures, the ratio of leukemic to normal granulocytic cells rose to about the same level, 6/1-7/1, just before granulopoiesis was suppressed. In both instances macrophage numbers were similar to those observed in control cultures of normal marrow cells alone. When mixtures of leukemic and normal bone marrow cells were assayed by the agar and spleen colony-forming techniques rather than by culture in DC, the formation of colony-forming cells in vitro was unaltered but there was a marked reduction in the formation of normal, differentiated spleen colony-forming cells.

• Research Organization: Beth Israel Hospital, Boston
• OSTI ID: 7208168
• Journal Information: Blood; (United States), Journal Name: Blood; (United States) Vol. 50:5; ISSN BLOOA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BLOOD FORMATION
BODY FLUIDS
BONE MARROW CELLS
CFU
CONNECTIVE TISSUE CELLS
DISEASES
GROWTH
HEMIC DISEASES
INHIBITION
LEUKEMIA
LEUKOCYTES
MAMMALS
MICE
NEOPLASMS
RADIATION EFFECTS
RADIATIONS
RODENTS
SOMATIC CELLS
TUMOR CELLS
VERTEBRATES