Expression of radiation-induced mutations at the arginine permease (CAN1) locus in Saccharomyces cerevisiae. [X radiation, uv radiation]

Journal Article · Mon Jan 01 00:00:00 EST 1979 · Genetics; (United States)

OSTI ID:6312128

Gocke, E; Manney, T R

In the yeast Saccharomyces cerevisiae, the expression of resistance to the L-arginine analog, L-canavanine, after mutagenesis, is strongly dependent on the metabolic state of the cell. The frequency of mutations recovered after exposure to ultraviolet light or x rays was measured under a variety of culture conditions. The results indicate that the frequency of mutants recovered is determined by the following three factors. The potential mutants still possess enough permease activity to take up some of the cell poison, and some are therefore killed before they can express the mutant genotype. The sensitivity is strongly influenced by the endogenous free arginine, which is in turn influenced by the growth medium. The rapid decay of the permease molecules and the inability of the potential mutants to resynthesize this protein results in a rapidly increasing chance of expression when selection is delayed. During the time when the permease activity is decaying, repair of the mutagen-induced damage appears to occur.

• Research Organization: Kansas State Univ., Manhattan
• OSTI ID: 6312128
• Journal Information: Genetics; (United States), Vol. 91:1
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
MUTATIONS
RADIOINDUCTION
SACCHAROMYCES CEREVISIAE
BIOLOGICAL RADIATION EFFECTS
ENZYMES
METABOLISM
MUTATION FREQUENCY
ULTRAVIOLET RADIATION
X RADIATION
BIOLOGICAL EFFECTS
ELECTROMAGNETIC RADIATION
FUNGI
IONIZING RADIATIONS
MICROORGANISMS
PLANTS
RADIATION EFFECTS
RADIATIONS
SACCHAROMYCES
YEASTS
560131* - Radiation Effects on Microorganisms- Basic Studies- (-1987)
550400 - Genetics