CAN1 locus of Saccharomyces cerevisiae: fine-structure analysis and forward mutation rates. [X radiation, uv radiation]

Journal Article · Mon Jan 01 00:00:00 EST 1979 · Genetics; (United States)

OSTI ID:6355052

Whelan, W L; Gocke, E; Manney, T R

A system of strains and growth media was developed to allow efficient detection of forward mutation, reversion, complementation, and suppression at the canavanine-resistance (CAN1) locus of Saccharomyces cerevisiae. Genetic fine-structure analysis revealed that the map length is at least 40, and possibly as much as 60 x-ray map units; this is the longest gene map yet reported in S. cerevisiae. Allelic complementation was not observed, despite testing of a large number of allele pairs, and alleles suppressible by the ochre suppressor SUP11 were absent from a sample of 48 spontaneous mutants and occurred infrequency (7%) among a sample of ultraviolet-induced mutants. Infrequent mutant types included canavanine-resistant mutants capable of arginine uptake and alleles thought to represent deletions or inversions. In contrast to previous reports in the literature, the spontaneous forward mutation rate at CAN1 did not increase during meiosis.

• Research Organization: Case Western Reserve Univ., Cleveland, OH
• OSTI ID: 6355052
• Journal Information: Genetics; (United States), Vol. 91:1
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
MUTATIONS
RADIOINDUCTION
SACCHAROMYCES CEREVISIAE
BIOLOGICAL RADIATION EFFECTS
ELECTRON MICROSCOPY
GENES
GENETIC MAPPING
ULTRAVIOLET RADIATION
X RADIATION
BIOLOGICAL EFFECTS
ELECTROMAGNETIC RADIATION
FUNGI
IONIZING RADIATIONS
MICROORGANISMS
MICROSCOPY
PLANTS
RADIATION EFFECTS
RADIATIONS
SACCHAROMYCES
YEASTS
560131* - Radiation Effects on Microorganisms- Basic Studies- (-1987)
550400 - Genetics