CAN1 locus of Saccharomyces cerevisiae: fine-structure analysis and forward mutation rates. [X radiation, uv radiation]

Journal Article · Mon Jan 01 04:00:00 EST 1979 · Genetics; (United States)

OSTI ID:6355052

Whelan, W L; Gocke, E; Manney, T R

A system of strains and growth media was developed to allow efficient detection of forward mutation, reversion, complementation, and suppression at the canavanine-resistance (CAN1) locus of Saccharomyces cerevisiae. Genetic fine-structure analysis revealed that the map length is at least 40, and possibly as much as 60 x-ray map units; this is the longest gene map yet reported in S. cerevisiae. Allelic complementation was not observed, despite testing of a large number of allele pairs, and alleles suppressible by the ochre suppressor SUP11 were absent from a sample of 48 spontaneous mutants and occurred infrequency (7%) among a sample of ultraviolet-induced mutants. Infrequent mutant types included canavanine-resistant mutants capable of arginine uptake and alleles thought to represent deletions or inversions. In contrast to previous reports in the literature, the spontaneous forward mutation rate at CAN1 did not increase during meiosis.

• Research Organization: Case Western Reserve Univ., Cleveland, OH
• OSTI ID: 6355052
• Journal Information: Genetics; (United States), Journal Name: Genetics; (United States) Vol. 91:1; ISSN GENTA
• Country of Publication: United States
• Language: English

Related Subjects

550400 -- Genetics
560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
ELECTROMAGNETIC RADIATION
ELECTRON MICROSCOPY
FUNGI
GENES
GENETIC MAPPING
IONIZING RADIATIONS
MICROORGANISMS
MICROSCOPY
MUTATIONS
PLANTS
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
ULTRAVIOLET RADIATION
X RADIATION
YEASTS