Effect of genes controlling radiation sensitivity on chemically induced mutations in Saccharomyces cerevisiae. [UV radiation]

Journal Article · Tue Jun 01 04:00:00 EDT 1976 · Genetics; (United States)

OSTI ID:7347523

Prakash, L

The effect of 16 different genes (rad) conferring radiation sensitivity on chemically induced reversion in the yeast Saccharomyces cerevisiae was determined. The site of reversion used was a well-defined chain initiation mutant mapping in the structural gene coding for iso-1-cytochrome c. High doses of EMS and HNO/sub 2/ resulted in decreased reversion of cyc1-131 in rad6, rad9 and rad15 strains compared to the normal RAD + strains. In addition, rad52 greatly decreased EMS reversion of cyc1-131 but had no effect on HNO/sub 2/-induced reversion; rad18, on the other hand, increased HNO/sub 2/-induced reversion but did not alter EMS-induced reversion. When NQO was used as the mutagen, every rad gene tested, except for rad18, had an effect on reversion; rad6, rad9, rad15, rad17, rad18, rad22, rev1, rev2, and rev3 lowered NQO reversion while rad1, rad2, rad3, rad4, rad10, rad12, and rad16 increased it compared to the RAD + strain. The effect of rad genes on chemical mutagenesis is discussed in terms of their effect on uv mutagenesis. It is concluded that although the nature of the repair pathways may differ for uv- and chemically-induced mutations in yeast, a functional repair system is required for the induction of mutation by the chemical agents NQO, EMS, and HNO/sub 2/.

• Research Organization: Univ. of Rochester, NY
• OSTI ID: 7347523
• Journal Information: Genetics; (United States), Journal Name: Genetics; (United States) Vol. 83:2; ISSN GENTA
• Country of Publication: United States
• Language: English

Related Subjects

560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOMASS
ELECTROMAGNETIC RADIATION
EMS
ENERGY SOURCES
ESTERS
FUNGI
GENES
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
INORGANIC ACIDS
MICROORGANISMS
MUTAGENESIS
MUTAGENS
MUTATIONS
NITROUS ACID
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PLANTS
PYRIDINES
QUINOLINES
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY
RENEWABLE ENERGY SOURCES
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
SULFONIC ACID ESTERS
ULTRAVIOLET RADIATION
YEASTS