Ultraviolet-induced reversion of cyc1 alleles in radiation-sensitive strains of yeast. III. rev3 mutant strains

Journal Article · Fri Jun 01 04:00:00 EDT 1979 · Genetics; (United States)

OSTI ID:5298934

Lawrence, C W; Christensen, R B

The role of the REV3 gene function in uv-induced mutagenesis in the yeast Saccharomyces cerevisiae has been examined by determining the reversion of 12 well-defined cyc1 mutations in diploid strains homozygous for the rev3-1 or rev3-3 allele. The 12 cyc1 alleles include one ochre, one amber, four initiation, two proline missense, and four frameshift mutations. We find that the rev3 mutations reduce the frequency of uv-induced reversion of all of the cyc1 alleles, though different classes of alleles respond to a different extent. These results imply that the REV3 gene function is required for the production of a wide variety of mutational events, though probably not all, and show that each of the three REV loci have different mutational phenotypes. Such diverse phenotypes are not predicted by the unitary model for bacterial mutagenesis, suggesting that this is at best an imcomplete description of eukaryotic mutagenesis.

• Research Organization: Univ. of Rochester, NY
• OSTI ID: 5298934
• Journal Information: Genetics; (United States), Journal Name: Genetics; (United States) Vol. 92; ISSN GENTA
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550400 -- Genetics
560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
DATA
DATA FORMS
ELECTROMAGNETIC RADIATION
EXPERIMENTAL DATA
FUNGI
GENES
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
GENOME MUTATIONS
INFORMATION
ISOLATED VALUES
MICROORGANISMS
MUTAGENESIS
MUTATION FREQUENCY
MUTATIONS
NUCLEOTIDES
NUMERICAL DATA
ORGANIC COMPOUNDS
PHENOTYPE
PLANTS
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RADIOSENSITIVITY
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
ULTRAVIOLET RADIATION
YEASTS