Ultraviolet-induced reversion of cyc1 alleles in radiation sensitive strains of yeast. II. rev2 mutant strains

Journal Article · Sun Oct 01 04:00:00 EDT 1978 · Genetics; (United States)

OSTI ID:6504008

Lawrence, C W; Christensen, R B

The range of specificity of the rev2-1 mutation, an allele that reduces the frequency of ochre revertants induced by uv in Saccharomyces cerevisiae, has been investigated by examining its influence on the reversion of eleven well-defined and contrasting cyc1 mutations. We have shown that the REV2 gene product is concerned only with the reversion of ochre alleles; it plays virtually no role in the reversion of amber, missense or frameshift mutations. We have also shown that its effect is specific and confined to only some highly revertible ochre alleles. The REV2 gene product appears to enhance reversion at these sites by facilitating the conversion of two otherwise nonmutagenic photoproducts into a single premutational lesion. UV-induced killing of rev2-1 strains was found to be significantly greater on fermentable rather than on nonfermentable media.

• Research Organization: Univ. of Rochester, NY
• OSTI ID: 6504008
• Journal Information: Genetics; (United States), Journal Name: Genetics; (United States) Vol. 90:2; ISSN GENTA
• Country of Publication: United States
• Language: English

Related Subjects

560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CELL KILLING
ELECTROMAGNETIC RADIATION
FUNGI
GENES
MICROORGANISMS
MUTATIONS
PLANTS
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
ULTRAVIOLET RADIATION
YEASTS