Fractionation of mutagens from municipal sludge and waste water
Technical Report
·
OSTI ID:6296238
There are potential environmental concerns from the disposal of municipal waste-water effluents and sewage-treatment-plant sludges. The report summarizes the microbial mutagenic evaluation of 13 sewage-sludge samples from various locations in Texas and Washington state. The sewage sludge samples were air-dried followed by sequential Soxhlet extraction with pentane, methylene chloride, and methanol. The organic extracts from three of the samples were further fractionated by normal phase high-pressure liquid chromatography (HPLC). The obtained extracts and fractions were bioassayed for microbial mutagenic response using the standard histidine reversion assay with Salmonella typhimurium strains TA98 and TA100, both with and without S9 metabolic activation. Extracts and fractions were chemically analyzed by high resolution gas chromatography (GC) using a variety of element-specific detectors, gas chromatography/mass spectrometry (GC/MS), and liquid chromatography/mass spectrometry (LC/MS).
- Research Organization:
- Pacific Northwest Lab., Richland, WA (USA)
- OSTI ID:
- 6296238
- Report Number(s):
- PB-89-161491/XAB
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATION
320604* -- Energy Conservation
Consumption
& Utilization-- Municipalities & Community Systems-- Municipal Waste Management-- (1980-)
520200 -- Environment
Aquatic-- Chemicals Monitoring & Transport-- (-1989)
54 ENVIRONMENTAL SCIENCES
550900 -- Pathology
59 BASIC BIOLOGICAL SCIENCES
ALKANES
BACTERIA
BIOASSAY
EVALUATION
EXTRACTION
FRACTIONATION
HYDROCARBONS
HYDROGEN COMPOUNDS
LIQUID WASTES
MASS SPECTROSCOPY
METHYLENE CHLORIDE
MICROORGANISMS
MUNICIPAL WASTES
MUTAGEN SCREENING
MUTAGENS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
OXYGEN COMPOUNDS
PENTANE
SALMONELLA
SAMPLING
SCREENING
SEPARATION PROCESSES
SEWAGE
SEWAGE SLUDGE
SLUDGES
SOLVENT EXTRACTION
SPECTROSCOPY
WASTE WATER
WASTES
WATER
320604* -- Energy Conservation
Consumption
& Utilization-- Municipalities & Community Systems-- Municipal Waste Management-- (1980-)
520200 -- Environment
Aquatic-- Chemicals Monitoring & Transport-- (-1989)
54 ENVIRONMENTAL SCIENCES
550900 -- Pathology
59 BASIC BIOLOGICAL SCIENCES
ALKANES
BACTERIA
BIOASSAY
EVALUATION
EXTRACTION
FRACTIONATION
HYDROCARBONS
HYDROGEN COMPOUNDS
LIQUID WASTES
MASS SPECTROSCOPY
METHYLENE CHLORIDE
MICROORGANISMS
MUNICIPAL WASTES
MUTAGEN SCREENING
MUTAGENS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
OXYGEN COMPOUNDS
PENTANE
SALMONELLA
SAMPLING
SCREENING
SEPARATION PROCESSES
SEWAGE
SEWAGE SLUDGE
SLUDGES
SOLVENT EXTRACTION
SPECTROSCOPY
WASTE WATER
WASTES
WATER